. Determine how many amino acids will appear on the protein encoded in a DNA segment that contains of about 981 nucleotide units. Show computation. Note: Assume that the entire segment is used to code for the protein and there are no start/stop codes present. 3. The human brain natriuretic peptide (BNP) is a small peptide of 32 amino acids used in the treatment of congestive heat failure. Determine how many nitrogen bases are present in the DNA that codes for this peptide. You may include the “start” and “stop” nitrogen bases. 4. Analysis showed that the base mole percent of A in sheep’s DNA is 29.3. Using Chargaff’s rule, calculate the base mole percent of the other bases. Finally, present your answers using a table containing the values computed.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
2. Determine how many amino acids will appear
on the protein encoded in a DNA segment that
contains of about 981
computation.
Note: Assume that the entire segment is used to
code for the protein and there are no
start/stop codes present.
3. The human brain natriuretic peptide (BNP) is a
small peptide of 32 amino acids used in the
treatment of congestive heat failure. Determine
how many nitrogen bases are present in the
DNA that codes for this peptide. You may
include the “start” and “stop” nitrogen bases.
4. Analysis showed that the base mole percent of A
in sheep’s DNA is 29.3. Using Chargaff’s rule,
calculate the base mole percent of the other
bases. Finally, present your answers using a
table containing the values computed.
5. Consider the following DNA template strand:
3’ GCA- AAA-CAA-ATA-GTG 5’
Using the following sequence, identify:
a. The base sequence in the DNA information
strand
b. Assuming that no introns are present, identify
the codons present in the mRNA transcribed
from the DNA template strand
c. The anticodons in the tRNA that with interact
with the mRNA codons in (b).
d. The amino acids that will be carried by the
tRNA (from c) molecules
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