A) Using this rule, determine the percentages of all the bases in DNA that is 20% thymine. [A] = [C] =_ [G] =. [T] = 20% B) If a single strand of RNA is 20% uracil, what can you predict about the percentages of the remaining bases and why?
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![29.)
equalities now called Chargaff's rule.
Biochemist Erwin Chargaff was the first to note that, in DNA, [A]=[T] and [G]=[C],
A) Using this rule, determine the percentages of all the bases in DNA that is 20% thymine.
[A] =
[C] =
[G]
[T] = 20%
%3D
-
B) If a single strand of RNA is 20% uracil, what can you predict about the percentages of
the remaining bases and why?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fddc59b4a-7887-4a90-8634-472f718e2168%2F09cb1376-139c-46b8-8922-2b252d719fb2%2F35a9ns_processed.jpeg&w=3840&q=75)
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- The melting temperature Tm of DNA can be predicted by calculation without actually measuring it. Calculate the Tm of the DNA double strand shown in (1) to (3), and discuss the results. The numbers in parentheses indicate the degree of polymerization of nucleotides.(1) A(10) + T(10), (2) A(15) + T(15), (3) G(10) + C(10)Name a.) ( pair. Indicate hydrogen bonds with dashed lines, number the atoms in the bases according to IUPAC convention, and circle the pyrimidine. Additionally, indicate where the sugar is attached to the base, the major groove, and the minor groove. 8.) Looking at the bases from above, draw a Watson-Crick C-G base b.) ( triple helix (shown below). In this triple helix, a third strand binds in the major groove via base pairs with the existing Watson-Crick bases. This additional base pair is called a Hoogsteen base pair and is indicated by an asterisk followed by the third base. On your drawing add a cytosine in the appropriate location to indicate a CG*C base pair. , it is possible for nucleic acids to form a structure known as a c.) ( between turns, glycosidic bond conformation and sugar conformation differs between A-form DNA and Z-form DNA, Which one is PNA? Indicate (possibly using a table) how the strand width, distanceBiochemist Erwin Chargaff was the first to note that, in DNA, [A] = [T] and [G]= [C], equalities now called Chargaff’s rule. Using this rule, determine the percentages of all the bases in DNA that is 20% thymine.
- 1- Biochemist Erwin Chargaff was the first to note that, in DNA, [A] = [T] and [G] = [C], equalities now called Chargaff’s rule. With the use of this rule, determine the percentages of all the bases in a DNA molecule which contains 35% thymine. Explain. 2- Similar equalities (i.e. [A]=[U] and [G]=[C]) are however not observed in RNA molecules. Explain the structural differences which dictate why Chargaff rule does not apply to RNA polynucleotides.131.) How many different sequence possibilities are there for a double-stranded RNA polymer that is 6 bases long? A) 64=(25) B) 192= (3x 25) C) 1296= (6) D) 4096= (45) E) 8192= (2x45)pppApCpCpUpApGpApU-OH(a) Using the straight-chain sugar convention, write the structure of the DNA strand that encoded this short stretch of RNA.(b) Using the simplest convention for representing the DNA base sequence, write the structure of the nontemplate DNA strand.
- For some DNAs, it is possible to separate the two strands, after denaturation,in a CsCl gradient.(a) What property of any DNA determines where it will band in a CsClgradient?(b) What kind of DNA might have two strands that differ sufficiently in thisproperty that they could be separated after denaturation?Bearing in mind the different number of hydrogen bonds that form between the two different purine- pyrimidine pairs in DNA, how would you explain the fact that DNA that is rich in cytosine-guanine pairs requires heating to a slightly higher temperature in order to separate the strands than DNA that is rich in adenine-thymine pairs?(a) (b) (c) The figure shown below is the mechanism of a DNA nuclease. The DNA molecule is in blue while the protein residues are in black (M1 and M2 are metal ions, they are irrelevant to this question). 3' Base reaction. M² 5' 00 3' Glu 357 M¹ Draw the structure of the final DNA product for this reaction. Draw a square around the nucleophile and draw a circle around the leaving group in this Does this depict the reaction of an endonuclease or an exonuclease?
- . A viral DNA is analyzed and found to have the following base com- position, in mole percent: A = 32, G = 16, T = 40, C = 12. (a) What can you conclude about the structure of this DNA? (b) What kind of secondary structure do you think it would have?For some DNAS it is possible to separate the two strands, after denaturation, in a CsCl gradient. (a) What property of any DNA determines where it will band in a CsCI gradient? (b) What kind of DNA might have two strands that differ sufficiently in this property that they could be separated after denaturation?. In a supercoiled DNA, a stretch of about 20 base pairs changes from the B form to the Z form. What is the change in (a) T, (b) L, and (c) W?