2. Spectrophotometry Assignment

Calculate the number of replicate analyses

Using data from Table 1 plot a graph similar to Figure 4 in the manual that you will label as “Figure A - Bromophenol Blue Stock Solution Absorbance Spectrum”. Remember all graphs need x-y-axis labels and titles. Plot the graph of order 2 polynomial as the best fit curve. The best-fit curve should show the equation of the line, it does not need to pass through the origin (0,0).

PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.

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Use the simulation lab to obtain the distance for the solvent front and the distance for each spot and then calculate the Rf 35ig figs acetaminoph Unknown Unknown Sample caffeine ibuprofen aspirin en Mixture #1 Mixture #2 13.0 cm13.0 cm 13. O cm 13.0 cm 13.0cm Distance to 130 cm solvent front 1.77cm 1.89cm 11.1 12.6 cm 1.78cm la.6 cm 11.0 cm 6.27cm Distance Spot(s) traveled 5.58cm Calculated R:(s) Components of Unknowns D. Additional Exercises 1. Which of the substances tested is most polar? What parts of the structure of this substance is polar? 2. Which of the substance tested is most non-polar? What parts of the structure of this substance in non-polar?

Using the data provided please help me answer this question.  Determine the concentration of the iron(Ill) salicylate in the unknown directly from to graph and from the best fit trend-line (least squares analysis) of the graph that yielded a straight line.

Just part b!!!

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Data Collection Kinetic Data Collection Time, t (s) Volume of added base (mL) First addition 40 0.50 Second addition 101 1.00 Third addition 160 1.52 Fourth addition 224 1.99 Fifth addition 278 2.49 Sixth addition 334 3.01 Seventh additlon 389 3.48 Calculations Concentration of NaOH stock solution: 0.01 M Concentration of t-BuCl stock solution: 0.2 M Volume of t-BuCl solution: 10 mL stock stock solution added to 25 mL acetone Table view List view Kinetic Data Volume of Moles of Amount of t-Bucl (mol) [-Buc] In[-BuC Time, t (s) added base added base (mL) (mol) First addition 40 0.50 Second additlon 101 1.00 Third addition 160 1.52 Fourth addition 224 1.99 Flfth additlon 278 2.49 Sixth additlon 334 3.01 Seventh addition 389 3.48 Use the data in the table above to plot In[t-BuC]]; vs time (s). Include labelled axis, a title, and a linear trendline. Upload your plot here.

Please do 16. Thank you.

Suzy Baker What three areas are affected by an "irritant"? (refer to ACS Safety in the Academic Chemistry Laboratories Vol. 1)    [use the answer format= ans 1, ans 2, ans 3]

NaOH is the base (please elaborate) Thank you!

I need help finding the %Composition for %cychohexane and %toluene. The formula (area of peak/total area) * 100%

sub= 18 help

Order of Most Linear Plot and Slope of linear Trendline

Question:  Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.  -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.

Not exactly sure how to fill out the table?

I need help with my homework

Please create a caption for this table.  Solution NaCl Conc. (%) Osmolality (mOsm)  % transmittance Absorbance % hemolysis % crenation C distilled  0 0 0.001029 4.987584625 100 0.03354 1 0.177179111 54.61 0.001551 4.809388202 96.42720001 0.05837 2 0.297126222 91.58 0.01012 3.994819487 80.09527231 0.08444 3 0.442542222 136.4 3.849 1.414652089 28.3634704 0.134 4 0.590164444 181.9 64.8 0.188424994 3.777880643 0.2125 5 0.74752 230.4 95.64 0.019360433 0.388172513 0.3368 6 0.89644 276.3 99.56 0.001915112 0.038397585 0.5336 7 1.095648889 337.7 99.98 8.68676E-05 0.001741676 0.9834 8 1.336711111 412 100 0 0 2.1 9 1.755568889 541.1 100 0 0 7.9 10 2.674395556 824.3 100 0 0 57.83 11 4.490211111 1384 100 0 0 99.72

50 Percentage Red No. 40 Soln 100% 80% 60% 40% Table 4.1: Dilutions of Red No. 40 & Absorbance Volume of Red No. 40 Soln (mL) 10me 8mL опи 4mL Percentage Red No. 40 Soln 100% 80% 60% 40% 20% Volume of DI Water (mL) OML 2mL LIML M UML 8ML Table 4.2: Dilutions of Red No. 40 & Absorbance Concentration Red No. 40 2. Report the wavelength with the maximum absorbance of Red No. 40 dye. Absorbance 20% 2ML Part A: Making a Calibration Curve for Beer's Law 1. Calculate the concentrations of each of your diluted Red No. 40 solutions and add these to Table 4.2. Show at least one sample calculation in the space below. 0.251 0.192 Total Volume 0.181 0·119 0.060 (mL) 10 mL 10 mL 10 mL 10 mL 10 mL

20,18,2,4,15,15,10 Using these data, construct a 80% confidence interval for the average net change in a student's score after completing the course. Assume the population is approximately normal.  Construct the 80% confidence interval. Round your answer to one decimal place.

Subpart 1-5

Answer in a detailed paragraph