Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
Calculate the number of replicate analyses
Transcribed Image Text:Concentration of stock protein solution (mg/mL): 0,4905
Unknown number:
Labquest-SpectroVis instrument number:
Standard
Concentration
Concentration of
Diluted Unknown
Sample
Absorbance Corrected Absorbance*
(mg/mL)
(mg/mL)
O.)3)
0.338
0.568
0.892
0.994
38
0.421
0.466
0,438
*Corrected absorbance is (Absorbance – Blank Absorbance).
Blank
Standard 1
0.207
0.237
0.761
0.863
1,00
0,290
0,335
0,307
Standard 2
Standard 3
Standard 4
Standard 5
Unknown
Unknown
1st replicate
2nd replicate
3rd replicate
Unknown
Observations (Submit via LON-CAPA)
Data Analysis
Slope
Y-intercept
Correlation coefficient
(3 sig. figs., SF = sig. fig.)
(3 SF)
(4 SF)
(3 SF)
2.09
0158
0.9889
0,0657
%3D
Standard error in y (oy)
Number of standard solutions
Number of replicate analyses
Sum of squares of deviation E(X; - X)²
Mean of y-values for standard solutions (Y std)
(3 SF)
(3 SF)
Average unknown protein concentration reporting value (mg/mL; diluted)
Uncertainty value for unknown protein concentration (Sx), (mg/mL; diluted)
Average unknown protein concentration reporting value (ppm; undiluted)
Uncertainty value for unknown protein concentration (ppm, undiluted)
Confidence interval based on the uncertainty value, (ppm, undiluted)
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