LEAD Cumulative Quiz 1
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Chemistry 4600 - Fundamentals of Biochemistry
LEAD Cumulative Quiz #1 September 25, 2022
Version A
This exam is closed book. The use of a non-programmable calculator is allowed. The time for
this exam is 50 minutes. Please use only the space provided for your answers and remember to
show all work to receive credit. There are a total of 110 points on this test. Your score will be out
of 100 points.
10 points will be extra credit
1.
5 pts. My research student treated cells with insulin in the lab. She saw no response. When
she looked back over the instructions, she noticed that the instructions call for treating cells
with 8 nM insulin. She used 4.5 x 10
-5
mg/mL insulin. Did she use enough? Prove it. The
molecular weight of insulin is 5808 g/mol
2.
Axitinib is a drug that is used for treatment of kidney cancer. The structure of axitinib is
shown below. Absorption of Axitinib in the stomach depends on its charge. Charged
molecules absorb better than uncharged molecules.
pKa= 4.58
a.
5 pts. Use the Henderson-Hasselbalch equation to determine the ratio of
charged to uncharged Axitinib at pH 2.5
b.
4 pts. Proton pump inhibitors are used to treat acid reflux and work by inhibiting
stomach acid secretion. They can increase the pH of the stomach to 5. Would
more axitinib be absorbed in the presence or absence of proton pump inhibitors?
c.
4 pts.Does axitinib contain an amide functional group? If so, circle on the
molecule above.
3.
Phosphohexose isomerase is a glycolytic enzyme that reversibly converts glucose
6-phosphate to fructose 6-phosphate. The production of fructose 6-phosphate has a ∆G˚’ of
+1.67 kJ/mol at 25 C.
Glucose 6-P
⇌
Fructose 6-P
a.
5 pts. Calculate the equilibrium constant for this reaction at 25 C.
b.
5 pts. ∆G for this reaction in the body (37 C) is -2.9 kJ/mol. Determine the ratio of
fructose 6-P to glucose 6-P under these conditions
c.
3 pts. Based on your answers above, which best explains why the reaction is
endergonic under standard conditions and exergonic under physiological
conditions?
-
The concentration of fructose 6-P is maintained below its equilibrium
concentration.
-
The concentration of glucose 6-P is maintained above its equilibrium
concentration.
-
The concentration of glucose 6-P is maintained below its equilibrium
concentration.
-
The concentration of fructose 6-P is maintained above its equilibrium
concentration.
4.
5 pts. Glycerol Kinase catalyzes the following spontaneous reaction:
Glycerol + ATP
⇌
Glycerol 3-Phosphate +ADP
Determine ∆G˚’ of the combined reaction using ∆G˚’ of it’s component reactions
-
Glycerol 3-Phosphate
⇌
Glycerol + Pi
∆G˚’ = -9.2 kJ/mol
-
ATP
⇌
ADP + Pi
∆G˚’ = -30.5 kJ/mol
5.
6 pts. Identify the following amino acids, include D or L in the name.
6.
5 pts. I am studying two proteins, Protein A and Protein B. Test tubes are prepared of each
protein at 2 mg/ml concentrations. Both proteins have 1 tyrosine amino acid in their primary
sequences. Protein A has 3 tryptophan amino acids in its primary sequence while Protein B
has 2 tryptophan amino acids in its primary sequence. Measuring the A
280
of my samples,
which one should have the higher absorbance? Why?
7.
12 pts. At what pH ranges, if any, would the peptides below exist as drawn?
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8.
Class II Alcohol Dehydrogenase (pi-ADH) has a pI of 8.6. This enzyme isolated from the
human liver facilitates the oxidation of ethanol to acetaldehyde while reducing NAD+ to
NADH.
a.
5 pts. Will pi-ADH have a net positive or net negative charge at pH 10? What
about pH 7?
b.
5 pts. At physiological pH, would we want to purify pi-ADH through anion
exchange chromatography or cation exchange chromatography?
c.
5 pts. I need to run my pi-ADH through dialysis to reduce my Tris buffer
concentration from 100mM to 8 mM. I have 25 mL of protein in my dialysis tube,
how much dialyzing buffer do I need?
d.
5 pts. I need my ADH to be at least 10 mg/mL so I let it run in the speed vac
overnight. I ran a Bradford assay and got the following standard curve. I had to
dilute my protein 3x to fit within the linear range and got an absorbance value of
0.182. Is my pi-ADH concentrated enough?
e.
5 pts. The ∆G˚’ for the oxidation of ethanol to acetaldehyde is 47.2 kJ/mol. At
37
℃
, what is the ∆G for this reaction given that we have 2.50 mM ethanol and
465 µM of acetaldehyde?
9.
6 pts. What amino acid produced the titration curve shown below? How many pKas are
present? How many pI’s?
10. 10 pts. Draw an antiparallel beta-sheet.
11. 5 pts. A given reaction is endothermic and has a positive ∆S. When will this reaction be
spontaneous?
a.
This reaction will always be spontaneous
b.
Low temperatures
c.
High temperatures
d.
This reaction cannot be spontaneous
e.
Not enough information given
12. 5 pts. We have learned that proteins are made of amino acid building blocks. Which
organelle is responsible for building proteins from amino acids?
a.
Mitochondria
b.
Golgi complex
c.
Rough Endoplasmic reticulum
d.
Smooth Endoplasmic reticulum
e.
Lysosyme
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Question 2
What is the pH of the solution which results from mixing 25 mL of 0.20 M CH3CO>H and 25 mL of 0.20 M NAOH? (K, for CH3CO>H = 1.8 x 10-b)
OA 8.87
OB. 10.43
OC7.00
OD.9 02
OE 5.13
A Moving to the next question prevents changes to this answer.
*-
F11…
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Instructions You are required to produce a short lab report using the information given in the accompanying document, ‘Lab report assessment_student data’. This document introduces an experiment, and also provides a set of experimental data for this experiment. You are required to use this data to perform steps 8 and 9 of the procedure. This includes calculations of a particular value, and then comparison to the true value. Consider any experimental errors when comparing calculated values and true values. Your report should be between 300 and 500 words (including all table contents and titles of tables and graphs). Your lab report should consist of three sections only: • Experimental • Results and Discussion • Conclusion You do NOT need to include an introduction section. Your experimental section should be written in appropriate lab report format, (passive voice, 3 rd person, in paragraphs). Your results and discussion section should contain the data presented in table format, and…
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Please answer questions 3 and 4 and show work please. Thank you
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omfg answer this
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The spectroscopic data in the table is generated with five solutions of known concentration.
Concentration (M)
0.0133
m=
0.0266
0.0532
0.106
0.213
Absorbance
0.1271
What is the intercept of the linear regression line?
0.08531
0.5388
1.069
Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line.
1.954
What is the slope of the linear regression line formed by these points?
M-1
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8 - 14 please
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rsity o...
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ARED4
ethod? 7...
F2
Remaining Time: 1 hour, 22 minutes, 23 seconds.
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Question 1
#
The angle of the water molecule (H₂O) is
180 degrees
120 degrees
100 degrees
90 degrees
109 degree
A Moving to the next question prevents changes to this answer.
MAR
14
80
F3
SA
$
000
F4
tv
%
F5
NA
MacBook Air
22
F6
∞r
F7
45.113
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ed Test
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Warnings appear when half the time, 5 minutes, 1 minute, and 30 seconds remain.
tiple Attempts Not allowed. This test can only be taken once.
ce Completion This test can be saved and resumed at any point until time has expired. The timer will
emaining Time: 57 minutes, 15 seconds.
uestion Completion Status:
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Question 4
The standard Gibb's Free Energy (AGO) = 18.38 kJ at 25C for the reaction
HNO2 2 H+ + NO2-.
What is the value of AG if {HNO2} = 0.75 M, {H+} = 0.13M and {NO2-} = 0.07M?
%3D
%3D
%3D
Is the reaction spontaneous under these conditions?
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+
terface/acellus_engine.htm?ClassID=1467683063
Use the data below to determine the
mass ratio of iron to oxygen for
compound 2.
Compound
1
2
Et
onal Academy of Science. All Rights Reserved.
Mass Fe
(grams)
15.55
20.98
[20:1
Enter the answer that belongs in the green box.
Iron Ratio Cmpd 2
Mass O
(grams)
4.45
9.02
Sh
Enter
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Dissolution
Distilled H20
Concentration
0.01 M HCI
Concentration
(mg/ml)
Time
Total amount
A
Total amount
A
(mg/ml)
(mg)
(mg)
5
0.16
0.1
10
0.24
0.18
15
0.35
0.25
20
0.48
0.34
25
0.58
0.4
30
0.67
0.5
1. Plot the given data on an ordinary paper and find the slope for each line then calculate the
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As you work on the questions, have your lab manual and text book handy.
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In the titration of 25.00 mL of a water sample, it took 19.090 mL of 3.165x 10-3 M EDTA solution to reach the
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6.04x10-5
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if you could please help! i submitted this previously and was told that it looks incomplete. the questions are complete and there is no additional information to upload with the questions. again there are 6 questions and i have done a majority of them. i really just need help with question 2 and 3, however, if you could look at my answers for the questions i did and tell me if they are correct i will gladly rate you! thank you so much. I apologize if it is small, i made the font smaller to fit everything on one picture
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Please help answer question 1, 2 and 3
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tes.You will be notified when time expires, and you may continue or submit.
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Remaining Time: 34 minutes, 54 seconds.
Question Completion Status:
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Question 11
The following question pertain to the charting of NMR spectra. MATCH the correct term to the description below.
The exact place on the chart at which a nucleus absorbs (i.e. where the peaks are located) is called its
high-field or upfield side
delta (6)
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МHZ
TMS
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