Spectrophotometry Post Lab

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Texas A&M University *

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120

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Chemistry

Date

Feb 20, 2024

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pdf

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2

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Spring 2023 CHEM 120 Chemical Kinetics Post-Lab Questions Make sure that your responses to these discussion questions is legible, if your responses are illegible they will not receive credit. We suggest that you type your responses. Using the data that you collected and wrote in your lab notebook during lab, organize your data into the required tables or graphs. Make sure that you format the table properly, i.e. include a title and labels for your columns, etc. Also make sure your plots are properly formatted and clearly labeled. 1. In a single table, tabulate your calibration curve data points. Make sure to include all proper labels and units for your solutions, concentrations, and Absorbance values. (2 points). Concentration (M) Absorbance 100% 0.154 50% 0.090 25% 0.056 12.5% 0.043 Unknown 0.065 750 NM 0.048 1. Attach your plot for your calibration curve. Please format your graph including a title, axis-labels and units. Include your best fit line displaying the appropriate slope, y-intercept and R 2 value. (2 points).
1. Should the trendline for the absorbance vs concentration plot be forced through the origin? Why or why not? Please provide your answer in paragraph form. (3 points) It should not be forced through the origin becaus eit would alter the slope drastically and it would also alter the y-intercept and r squared value as well. Also, absorbance cannot equal 0 because this means that no light was absorbed which is not true other than when you are calibrating the spectrophotometer. 1. If (0,0) was added to your data set, would that be equivalent to forcing the trendline through the origin? Why or why not? Please provide your answer in paragraph form. (3 points) No, it would not be the same because is you plugged in (0,0) into y = 427x+0.0257, you would get the same y-intercept. The trendline would not go through (0,0), it wuld be just like adding another data value.
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