Answered: Reaction velocity (µM · s-1) Substrate…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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You are trying to determine the K_M for an enzyme. Due to a lab mishap, you have only two usable data points: Use these data to calculate an approximate value for K_M. Is this value likely to be an overestimate or an underestimate of the true value? Explain.

Reaction velocity
(µM · s-1)
Substrate
concentration (µM)
1
5
100
50

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How did you get 0.32?

Also where did you get 500 for Vmax? In the question it says 50

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