PhysioEx Exercise 12 Activity 4

PhysioEx Lab Report Exercise 12: Serological Testing Activity 4: Western Blotting Technique Name: Jennifer Adams Date: 4 May 2022 Session ID: session-4a063bf3-b1c2-61f6-6677-a77ef8ae201f Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Questions No Predict Questions Stop & Think Questions Which of the following tests is not correctly matched to what it tests for? You correctly answered: These are all correctly matched. 1 Gel electrophoresis is used to separate proteins on the basis of You correctly answered: size and charge. 2 Western blotting can be used to test for You correctly answered: HIV and Lyme disease. 3 In this activity you will use Western blotting to test for the presence of You correctly answered: antibodies (like the indirect ELISA). 4 What is present on the nitrocellulose strips? You correctly answered: HIV antigens separated by electrophoresis. 1 How would you describe the binding site of the secondary antibody? 2

Experiment Data Sample gp160 gp120 p55 p31 p24 HIV Test Result Patient A no no no no no - Patient B no no yes no yes IND Patient C yes yes yes no yes + Positive Control yes yes yes yes yes + Negative Control no no no no no - Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Review Sheet Results You correctly answered: the constant region of the primary antibody. The patient samples being tested contain You correctly answered: serum. 1 The primary antibodies in the Western blot You correctly answered: come from the patient sample. 2 A key di ff erence between the ELISA and the Western blot technique is that the You correctly answered: Western blot looks at separated antigens. 3 The antibodies in a positive patient sample bind to the You correctly answered: constant region of the enzyme-linked antibody and the antigen bound to the nitrocellulose strip. 4 Describe why the HIV Western blot is a more speci fi c test than the indirect ELISA for HIV. Your answer: The HIV Western blot is more speci fi c than indirect ELISA for HIV because we are 1