PhysioEx Exercise 12 Activity 2

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Pepperdine University *

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Biology

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Jan 9, 2024

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PhysioEx Lab Report Exercise 12: Serological Testing Activity 2: Comparing Samples with Ouchterlony Double Di ff usion Name: Jennifer Adams Date: 4 May 2022 Session ID: session-150e3e65-8198-d127-dbe1-fd2d546e1019 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Question Stop & Think Question When two antigens are identical their precipitin lines form You correctly answered: an arc. 1 Antigen and antibody move toward each other because of You correctly answered: di ff usion. 2 If two antigens form a spur, they You correctly answered: have partial identity. 3 The Ouchterlony test relies on the formation of a(n) You correctly answered: precipitate. 4 Predict Question: How do you think human serum albumin and bovine serum albumin will compare? Your answer: They will have partial identity. 1 Which of the following best describes the two solutions you just added to well 1? 1

Experiment Data Wells Identity 2 and 5 Partial 2 and 3 Identity 3 and 4 Partial 4 and 5 Identity Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. You correctly answered: They are both antibodies produced by a goat. There is a spur between wells 2 and 5 and between wells 3 and 4 because the You correctly answered: antigens have partial identity. 1 What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)? You correctly answered: human serum albumin. 2 Which of the following best describes this test? 3

Review Sheet Results You correctly answered: Antibodies are used to detect the presence of antigens. The antigen-antibody precipitate forms where the ratio of antigen and antibody is You correctly answered: optimum. 4 Describe how you were able to determine what antigen is in the unknown well. Your answer: Since human serum albumin was in well #4 and wells 4 and 5 were identical, this meant that the unknown well (#5) was also human serum albumin. 1 Why does the precipitin line form? Your answer: A precipitin line forms when there is an equivalence between antibodies and antigens. 2 Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction? Your answer: I predicted that human serum albumin and bovine serum albumin will have partial identity. This prediction was accurate with the results of the expriment since a single spur formed. This indicates that they have epitopes in common. 3

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