Prelab 3

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University of Ottawa *

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BIO3126

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Biology

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Jan 9, 2024

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1

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Prelab 3 SIMPLE STAINS 1. What are three characteristics that the simple staining technique allow you to observe? (2 points) Allows us to observe size, shape and grouping or aggression of cells 2. A black marker (Sharpie) will be used to perform a negative stain of bacteria. Given the principal of this method, what must be the charge of the marker’s ink? (2 points) negative 3. What is the charge of the glass slides used in this lab? (2 points) Positive MICROSCOPY 4. Complete the following table to indicate which pictures of stained bacteria you will have to obtain in this lab: (2 points) Exercise N o Type of staining Source Magnification Number of images 3.4 Positive Soil (2.2) 1000X 1 3.4 Positive Body (2.5) 1000X 2 3.4 Positive Environmen t (2.5) 1000X 2 3.5 negative Soil (2.2) 1000X 1 STREAKING FOR SINGLE COLONIES 5. A single colony isolation is achieved by performing three independent sets of streaks on an agar plate. How many times, from start to finish must the inoculation loop be sterilized? (2 points) Should be sterilized 4 times. At the start before streaking, between streaks 1- 2, between streaks 2-3 , and at the end when we are finished.
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