Enzyme Activity Lab Report: Understanding Peroxidase Functions

L] Laboratory Report 4: Enzymes \ W e Ok Name: YAl Date 3y Points: Table # Lab day/time L ———————————————— D —————————— Activityl:1 (i ) \ Table1-1 [ Tube2 1ml peroxidase Total mL Color L ol 0 1. Whatis the pH of the peroxidase extract? {, of tube 27 2. What is the purpose of tube 17 What is the purpose \ \ Wesod S0 ophs p AN volumes of water in each tube? p Q 3, Why are there different Ty Vel Yoo\ o WD 4. What is the physical evid of peroxidase? ence that confirms the activity e CONOT 1-2 M L 1.0 ml peroxidase 0.5 mL guaiacol 1mL 1ml 1mL 5.5 mL somlL |Ludomb 2mb distilled Hz20 and the n of enzyme Color intensity etween the concentratior istence of a relationship b e relationship. (ONCR OO0 \Crense 5. Does your data support the ex 1f so, please describe th rate of the reaction? )X 0 TR 5 Y 4O

n would not continue to increase indefinitely as o Increnzed: WhY mighet i vort &~ zym r wnine 'nCrE Sed of the 5 be so? CcenraXion, t rea i or Table 1-3 — maable Zad Sl ey ~— AT (AR ey pH3 | pHS I i PHO Bl 1 e i | Y e ey B8 7. Do your data suggest that there is an optimal (best) pH for enzyme activity, and if so, what is it? Remember to graph your data and turn it in with this report. B 5 tne opymen (be s 8. Is this optimal pH similar to the pH of the plant extract? Please explain why this may or may not be s Lar P Ve S dooaek Ves, \becads e (X Aok NWaS - Table 1-4 plor e Estimated Actual temperature | temperature [ icewater | 070 Room temp. | (e [ o [ Boiling 00 "k | | 9, What happens to the peroxidase activity as you increase the temperature from 0°C through 37°C? e NCXEADE ! o (S Sy 10. Of all the temperatures tested, which is the optimal temperature for peroxidase activity? | & | 11, What happens to peroxidase activity above the optimal temperature and why? | AN S\ O INS | e\ 12. Do you think all enzymes will react similarly to the tests you performed in these a PWOAC WY why not? o Y Meg T *ee v ’ s3NTRONC MY 13. Are your data from Investigation 1 qualitative or quantitative? Please explain. B o e\ vo e JUO NNV

Name 10N Unit 4: Enzymes 4 Lab time L lmm \WPotesis & o 5 NRAIVE 14, What wys the purpose of Tube 1 in Activity 2-17 What was the purpose 2 FTule in Activ Se o Thbe 1 B ¥ oriing To SeL \ 15. What is your group’s assigned question about fungal lactase activity? A0Ls Sungo\ |addse normdly Sun n % 16. What is your hypothesis (educated guess) about this question? enzme \a se W Woete by ¢ MA ees TN BT 3V, " [Activity 2-3 g 17. What is the dependent variable of your experiment? Please review the introduction of activity 2-3 for a description of what the dependent and independent variables are. endent \( ot P& Yoo measure in my Ay des vy y § variable of your experiment? 18, What is the It e Chande , Sowe\e Vi Yhe CXPeriment ‘ 19, What is the purpose of your positive control? What is the purpose of your negative control? SERRNGE RN - o able X \ S table below. Please enter any additional ample, a different substrate, salt, etc): tween 3 to 5 samples. 20. Fill in the volumes of the reagents you will use in the reagent you might use in the blank space in the first column (for exa Depending on how you write your hypothesis, your group will run be _Table 2-3 i RE A A B Sample 1 sampleZ | Sample3 | Sample4 | SampleS | pos. control neg.control | test | st test \ | Lactase 0.1 ml pH buffer volume 0.9 mL | \ pH level 7 | ! M L e Temperature | Don't forge i B Milk TmL [ T “ __//_‘,//% Total volume Zml 2mL ZmL Zm 2 fi Result I I > ‘ \ ] s an alternative reagent to test, like salt or sucrose. * An empty row is provided in case your question add: M

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Activity 2-3. continued that should be included are: What order should reagents get mixed? Will enzyme be added immediately pre-incubation? At what temperatures will samples be kept? How long will samples be orafter incubated? Review the instructions in Activity 2-1 to see what your protocol should look like. /u What is your evidence for lactase function and how will you measure it? 23. What conclusion ¢ examined? 24. How do your data in Investigation 2 compare with your findings from Investigation 1? According to your data, do peroxidase and lactase function under similar conditions? Please explain. \as dor

lab 4

Related Documents