Bacterial-Identification-Lab-Worksheet-Student 2020 (1) (1)

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www.BioInteractive.org Published January 2015 Page 1 Student Handout Virtual Lab Bacterial Identification Virtual Lab BACTERIAL IDENTIFICATION LAB HANDOUT INTRODUCTION Go to https://www.hhmi.org/biointeractive/explore-virtual-labs . Scroll down and click on “The Bacterial Identification Virtual Lab.” Maximize the screen if you wish. Answer the following questions in the spaces provided . Click to Enter the Lab. (Click the window on the left-hand side of the screen to enter the lab.) As you enter the lab, follow the instructions in the lab (left-hand window). Using the information in the Notebook window on the right, answer the following questions. PART 1: SAMPLE PREPARATION 4. As the pathology lab technician, what is your task in this virtual lab? 5. Extracting DNA involves which initial step? 6. What is the wire ring used for? 7. Why are the proteolytic enzymes necessary?
www.BioInteractive.org Published January 2015 Page 2 Student Handout Virtual Lab Bacterial Identification Virtual Lab 8. Why do you then need to inactivate the proteolytic enzymes and how do you do it? 9. After removing the enzymes, why do you spin the sample in the centrifuge? 10. a. What is the pellet? b. What is the supernatant? c. Where is the DNA? PART 2: PCR AMPLIFICATION Go on to Part 2 and work through the PCR steps. Be sure to read the information in the notebook, including “What is PCR?” 11. What does “PCR” stand for and what is the purpose of PCR? 12. Summarize the process of PCR in a diagram. Include all the steps, labeled and in the right order. ( D raw the diagram on a piece of paper, take a photo, and upload it to your document .)
www.BioInteractive.org Published January 2015 Page 3 Student Handout Virtual Lab Bacterial Identification Virtual Lab Add the Master Mix and answer the following questions: 13. What does the Master Mix contain? 14. What are primers? Why is a primer added? 15. Once the primers bind, what occurs next? 16. What does "highly conserved" mean? 17. Why are highly conserved regions important in this lab? 18. What does "highly variable" mean? 19. Why are highly variable regions important in this lab? 20. What is missing in the negative control tube? 21. What is present in the positive control tube that is not in the negative control tube? Now run the PCR. Be sure to watch the virtual lab animation before proceeding to the questions . 22. List each step of a PCR cycle, the temperature, and the duration (time). a. b.
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www.BioInteractive.org Published January 2015 Page 4 Student Handout Virtual Lab Bacterial Identification Virtual Lab c. 23. Describe what happens during each of the steps in one or two sentences. a. b. c. 24. After eight cycles, how many copies of the desired DNA have been synthesized? 25. After 30 cycles?
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www.BioInteractive.org Published January 2015 Page 7

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