12/20/23, 1:24 AM
HW 9 Flashcards | Quizlet
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Oct4 is an embryonic stem cell specific
transcription factor. ESCs grown in petri dishes are
fed media that contain basic fibroblast growth
factor (bFGF) which keeps the ESCs
undifferentiated. They examine Oct4 mRNA levels in
human ESCs (hESCs), hESCs with exogenous miR-
223 (micro RNA 223), hESCs with exogenous miR-
223 that do not have bFGF in the media, and hESCs
without bFGF but with miR-223 inhibitor.
If extra miR-223 is added to the cells, this leads to
(MORE/LESS/THE SAME) Oct4 levels. We conclude
this based on which time points?
Less... 120 xxxx
Less 72xxxxxx
Third option Less 72 and 120???
Oct4 is an embryonic stem cell specific
transcription factor. ESCs grown in petri dishes are
fed media that contain basic fibroblast growth
factor (bFGF) which keeps the ESCs
undifferentiated. They examine Oct4 mRNA levels in
human ESCs (hESCs), hESCs with exogenous miR-
223 (micro RNA 223), hESCs with exogenous miR-
223 that do not have bFGF in the media, and hESCs
without bFGF but with miR-223 inhibitor.
Based on this data, what is the relationship between
miR-223 and ESC differentiation?
miR-223 levels and ESC differentiation are not relatedxxxx
miR-223 is important to keep ESCs undifferentiatedxxxx
miR-223 is important to keep ESCs undifferentiated only in the absence of
bFGF???
The sequence for a given mRNA is 5' AGGUAGC 3'.
If the genetic code was overlapping, which of the
following would be possible ways for the codons to
be read?
AGG UAG???
AGG GUA AGC???
AGG GUA UAG AGC?
AGG GGU GUA UAG?
Imagine the CCA added to the 3' end of the tRNA
consisted of DNA nucleotides. It (WOULD/WOULD
NOT) be possible to connect an amino acid to this
tRNA. Explain.
Would...xxxx
.because both are capable of phosphodiester bond formationxxx
Wouldnotxxxxx
Would???
..depends on the corresponding aminoacyl-tRNA synthetase???
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