Chapter 15 - Mo Bio Flashcards _ Quizlet

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 1/11 Chapter 15 - Mo Bio 9 studiers from Ryerson University 5.0 (2 reviews) Students also viewed Terms in this set (44) Try Magic Notes and save time Upload notes Molecular Bio Test #4 19 terms kgoldsborough1 Preview Ch 15 TopHat Teacher 15 terms william613 Preview Test Four Molecular Biology 312 144 terms leah_marie_sawyer Preview Molecular B 27 terms shelby_r Transcription is defined as: A. the production of a strand of RNA with a sequence identical to the DNA template. B. the production of a strand of DNA with a sequence identical to the RNA template. C. the production of an exact complementary strand of RNA on a DNA template. D. the production of an exact complementary strand of DNA on a RNA template. E. the production of an exact complementary strand of RNA on a RNA template. C. the production of an exact complementary strand of RNA on a DNA template. The general name for enzymes that catalyze RNA synthesis directed by a DNA template is: A. RNA synthetase. B. RNA polymerase. C. polynucleotide phosphorylase. D. RNase. E. DNA polymerase. B. RNA polymerase. 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 2/11 Which of the following characteristics of DNA- dependent DNA synthesis is not the same for DNA-dependent RNA synthesis? A. Synthesis requires a template. B. Initiation involves the recognition of a specific DNA sequence. C. Initiation of synthesis requires a primer. D. Synthesis is catalyzed in the 5' to 3' direction. E. All of the choices given are the same for both DNA and RNA synthesis. C. Initiation of synthesis requires a primer. Which of the following characteristics of transcription is the same for DNA replication? A. The process has distinct initiation, elongation, and termination phases. B. Just one of the two strands of a DNA segment serves as a template. C. Only certain genes serve as templates at any given time. D. Nascent strands dissociate from the template. E. All of the choices given are the same for both transcription and DNA replication. A. The process has distinct initiation, elongation, and termination phases. Which of the following is not true of the reaction catalyzed by RNA polymerases? A. Template DNA is copied in the 3' to 5' direction. B. The 3'-hydroxyl group of the growing RNA strand is a nucleophile. C. The reaction is magnesium ion-dependent. D. U residues are incorporated into the RNA to pair with T residues of the template. E. The RNA molecule is synthesized in the 5' to 3' direction. D. U residues are incorporated into the RNA to pair with T residues of the template. Which of the following eukaryotic RNA polymerases is responsible for transcribing nearly all protein-coding genes? A. RNA polymerase I B. RNA polymerase II C. RNA polymerase III D. RNA polymerase IV E. a complex of RNA polymerase II and III B. RNA polymerase II Learn 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 4/11 What is the correct order of transcriptional events occurring after RNA polymerase binds to the promoter? A. start of RNA synthesis, open complex formation, closed complex formation, promoter clearance B. closed complex formation, open complex formation, promoter clearance, start of RNA synthesis C. closed complex formation, open complex formation, start of RNA synthesis, promoter clearance D. open complex formation, closed complex formation, start of RNA synthesis, promoter clearance E. open complex formation, closed complex formation, promoter clearance, start of RNA synthesis C. closed complex formation, open complex formation, start of RNA synthesis, promoter clearance Which of the following molecules inhibits bacterial transcription by prevention of promoter clearance? A. Alpha-amanitin B. actinomycin D C. rifampicin D. acridine E. ethidium bromide C. rifampicin Which of the following does not contribute to the efficiency with which E. coli RNA polymerase binds to a promoter? A. the sequence of the upstream promoter element B. the binding of a sigma subunit C. the sequence at the +10 region D. the sequence at the -35 region E. the distance between the consensus sequence and the transcriptional start site C. the sequence at the +10 region What is the function of the SIGMA subunit of E. coli RNA polymerase in transcription? A. It contains the polymerase active site. B. It contains the separate exonuclease site required for proofreading. C. It directs the polymerase to bind a specific type of promoter sequence. D. It signals the polymerase to terminate transcription at specific sites. E. It allows template-independent RNA synthesis. C. It directs the polymerase to bind a specific type of promoter sequence. Learn 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 5/11 When does the SIGMA subunit dissociate from RNA polymerase? A. after promoter clearance B. at the termination of transcription C. immediately after promoter binding D. immediately before promoter binding E. not until the enzyme is degraded A. after promoter clearance Which of the following sigma factors is essential for general transcription in exponentially growing cells? A. σ 28 B. σ 32 C. σ 38 D. σ 54 E. σ 70 E. σ 70 Which of the following sigma factors would be expected to associate with RNA polymerase after increasing the incubation temperature of an E. coli culture from 30 ° C to 42 ° C? A. σ 28 B. σ 32 C. σ 38 D. σ 42 E. σ 54 B. σ 32 "DNA footprinting" is a technique that can be used to identify: A. a region of DNA that has been damaged by mutation. B. the position of a particular gene of a chromosome. C. the position of internally double-stranded regions in a single-stranded DNA molecule. D. the specific binding site of a repressor, polymerase, or other protein on the DNA. E. All of the choices given can be identified by this technique. D. the specific binding site of a repressor, polymerase, or other protein on the DNA. Learn 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 7/11 Which of the following is not true of the kinetic proofreading reaction of bacterial RNA polymerase? A. rNTP is the product. B. It occurs when the enzyme stalls after incorporating a mismatched base. C. The type of reaction is pyrophosphorolysis. D. Water is the nucleophile. E. Pyrophosphate is a reactant. D. Water is the nucleophile. Which of the following is not true of the nucleolytic proofreading reaction of bacterial RNA polymerase? A. Water is the nucleophile. B. It occurs when the enzyme backtracks after incorporating a mismatched base. C. rNTP is the product. D. It involves the melting of several RNA-DNA base pairs. E. All of the choices given are true. C. rNTP is the product. Which of the following is true of ρ -independent termination of transcription? A. It relies on the production of an RNA transcript with self-complementary sequences. B. It utilizes a hexameric helicase. C. RNA polymerase stalls at a specific sequence of GC base pairs. D. It occurs when the sigma subunit dissociates from the polymerase. E. It requires ATP hydrolysis. A. It relies on the production of an RNA transcript with self-complementary sequences. Which of the following transcription factors is utilized by all three eukaryotic RNA polymerases? A. TFIID B. TBP C. TFIIIC D. TFIIF E. TFIIIB B. TBP Which of the following RNA products are least likely to be synthesized by RNA polymerase II eukaryotic cells? A. noncoding RNAs B. microRNA C. mRNA D. tRNA E. None of the choices given is a RNA polymerase II product. D. tRNA Learn 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 8/11 Which of the following sequences is not generally found in the core promoter of RNA polymerase II? A. BRE B. DPE C. enhancer D. Inr E. TATA C. enhancer All of the following are characteristics of RNA polymerase III promoters except: A. the promoters contain two specific segments for transcription factor binding that lie within the coding sequences. B. the TATA box is for TFIIIB binding. C. promoter boxes A and B are bound by TFIIIC and lie downstream of the transcriptional start site. D. within a species, all core promoters are the same. E. All of the given characteristics are true of RNA polymerase III promoters. D. within a species, all core promoters are the same. Which of the following is not true of the interaction of TBP with DNA? A. TBP is able to interact with DNA nonspecifically with respect to sequence. B. TBP binds the TATA box through minor groove DNA contacts. C. A helix-turn-helix motif in TBP makes the DNA contacts. D. The DNA bound by TBP is bent. E. Two pairs of phenylalanine side chains are positioned between base pairs. C. A helix-turn-helix motif in TBP makes the DNA contacts. In which of the following steps of transcription is the C-terminal domain of RNA polymerase II first phosphorylated? A. assembly of the preinitiation complex B. initiation C. elongation D. termination complex formation E. after termination B. initiation Which of one the following amino acids is not phosphorylated in the C-terminal domain of DNA polymerase II? A. Phe B. Ser C. Tyr D. Thr E. All of the amino acids given are phosphorylated. A. Phe Learn 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 10/11 Which of the following is not a feature of the "torpedo" model for RNA polymerase II transcription termination? A. Conformational changes occur in the Pol II elongation complex when elongation factors dissociate. B. RNA is cleaved downstream from the poly(A) site. C. Exonuclease degradation of RNA promotes Pol II release. D. Pol II remains associated with the DNA template after mRNA synthesis. E. All of the choices given are a feature of the "torpedo" model. A. Conformational changes occur in the Pol II elongation complex when elongation factors dissociate. Which of the following models for RNA polymerase II transcription termination is analogous to ρ - dependent termination in bacteria? A. the antiterminator model B. the submarine model C. the torpedo model D. the allosteric model E. the hairpin model C. the torpedo model Which of the following is not a function of the general transcription factor, TFIIH? A. unwinding of the DNA near the transcriptional start site B. recruits nucleotide excision repair proteins to the site of a DNA lesion C. direct interaction with RNA polymerase II D. recruitment of TFIID to the promoter E. formation of the closed complex D. recruitment of TFIID to the promoter Which of the following statements regarding transcription only apply to eukaryotes and not to prokaryotes? A. A single RNA polymerase transcribes genes that encode mRNAs, tRNAs, and rRNA. B. Sigma () subunit detaches from RNA polymerase shortly after transcription has initiated. C. The C-terminal domain of the polymerase is phosphorylated after transcription has initiated. D. RNA synthesis is inhibited by actinomycin D. E. RNA synthesis is inhibited by rifampicin. C. The C-terminal domain of the polymerase is phosphorylated after transcription has initiated. Learn 4 New achievement earned! You hit a 4-day study streak

12/20/23, 1:23 AM Chapter 15 - Mo Bio Flashcards | Quizlet https://quizlet.com/243636530/chapter-15-mo-bio-flash-cards/ 11/11 Which of the following statements regarding transcription only apply to prokaryotes and not to eukaryotes? A. A region of the promoter about 30 nucleotides upstream of +1 contains AT-rich base pairs. B. The RNA start site is designated +1. C. A region of the promoter about 10 nucleotides upstream of +1 contains AT-rich base pairs. D. Important sequence elements are frequently found downstream of +1. E. All of the choices given apply to eukaryotes. C. A region of the promoter about 10 nucleotides upstream of +1 contains AT-rich base pairs. Which of the following characteristics of bacterial transcription is not true of eukaryotic transcription? A. Specific factors are used to direct the polymerase to bind a specific type of promoter sequence. B. Abortive transcripts are formed prior to the elongation phase. C. RNA polymerase interacts with a single transcription factor during initiation. D. RNA polymerase interacts with DNA upstream from the coding sequence in the template. E. Transcription has distinct initiation, elongation, and termination phases. C. RNA polymerase interacts with a single transcription factor during initiation. Which of the following processes is least likely to be coupled to eukaryotic transcription? A. DNA repair B. DNA replication C. RNA processing D. mRNA transport E. All of the choices given are coupled to eukaryotic transcription. B. DNA replication Learn 4 New achievement earned! You hit a 4-day study streak