Bio 200 Lab 9; Ashley Valencia

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Feb 20, 2024

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Bio 200 Early Plants Lab Kingdom Plantae - Supergroup Archeaplastida Objectives 1. To examine the diversity of the Kingdom Plantae 2. To Identify and learn the characteristics that distinguish Bryophyta, Marchantiophyta, and Monilophyta. Lab SLO Objective #3 3. To understand the alternation of generations life cycle for land plants. See page 618 of text. 4. To understand the key adaptations for the early plants Circle the correct response for each statement. Both Marchantiophyta and Bryophyta have a GAMETOPHYTE or SPOROPHYTE (circle) dominant life cycle. Monilophyta has a GAMETOPHYTE or SPOROPHYTE (circle) dominant life cycle. Marchantiophyta, Bryophyta, and Monilophyta are HOMO or HETERO sporous plants (circle). Marchantiophyta, Bryophyta, and Monilophyta have the eggs develop in the ANTHERIDIA or ARCHEGONIA (circle). Marchantiophyta, Bryophyta, and Monilophyta have the sperm develop in the ANTHERIDIA or ARCHEGONIA (circle). Phylum Marchantiophyta - liverworts (Ch 25.3) If available, observe the fresh material by using your dissecting scope. Otherwise use your text and watch the videos on Canvas to make sure you know what they look like. o What generation is the thallus? o What is its ploidy level? o What is the function of the pores? Look for some “half cups” filled with small pieces of the thallus nestled inside. These are known as gemmae (gemma = singular). If there is no fresh material, then watch the Canvas videos to see the gemmae. o If gemmae are clones of the thallus they come from, are they made asexually or sexually? Observe the preserved archegoniophore (archegonia= female reproductive area, phore = to bear) and antheridiophore (antheridia = male reproductive area, phore = to bear) of the liverwort, Marchantia , located in alcohol in a jar. Do not take them out, but realize these structures are what you are looking at on the slides. These structures are located on the thallus and are 1N. Draw and label the archegonia and antheridium slides of Marchantia . Eggs are found under the archegoniophore and sperm are found on top of the antheridiophore. Only one egg develops per archegonium. Use your textbook (Figure 25.11) to help with structure identification. o What cellular division produces the gametes in the gametangia?
o What is the ploidy level of the finished gametes? o Are the sperm flagellated or unflagellated? See text and Canvas videos for help. Draw and label the sporophyte slide of Marchantia . Each sporophyte capsule is attached with a foot and seta to the underside of the female archegoniophore. Inside the capsule you should find thin wavy lines that are parallel with the capsule length. These are elaters that twist around and help with spore dispersal. Use your textbook for help with structure identification. o On which gametophyte, male or female, should the sporophyte be found? o Inside the capsule are the spores. What is the ploidy level of the sporophyte structure? o What is the ploidy level of the finished spores? o What cellular division is used to make spores? Phylum Bryophyta - mosses (Ch 25.3) If available, observe the fresh material. Along with the gametophytes you might find thin green filaments growing amongst the gametophytes. These are protonemata (protonema = singular), which came from geminated spores, and they will eventually grow to look like the adult gametophytes. Rhizoids are found at the base; they are not roots. There may be sporophytes that stick upwards from some of the gametophytes. Draw and label the archegonia and antheridium slides of mosses. The gametangia are located at the top of the moss structures. Only one egg develops per archegonium. Use your text to help with structure identification. o What is the ploidy level of the moss gametophytes? o What cellular division produces the gametes? o What is the ploidy level of the finished gametes? o Are the sperm flagellated or unflagellated? See Canvas videos for help. Draw and label the sporophyte (only capsule portion) slide of a moss. You will need to move your slide around to see the whole structure at 40X. Use your text to help with structure identification. o What cellular division produces the spores? o What is the ploidy level of the finished spores? Phylum Monilophyta - ferns If available, observe the fresh fern material. It will be your representative plant from Monilophyta for the course. Draw and label the slide of the sorus (indusium) using your text to help with structure identification. The slide is a cross section through a portion of the leaflet with the sorus below. The indusium is a shield that holds the sorus against the leaflet, protecting the developing sporangia. o What are the spores ploidy level and what cellular division was used to make them? o What is the ploidy level of the leaflet?
Draw and label the fern gametophyte with archegonia and antheridium. Each gametophyte will produce BOTH gametangia, but not at the same time. The gametophyte is upside down; you are looking at the underside. The slide will have several gametophytes per slide. You will need to view one gametophyte to view archegonia and view a different gametophyte to view the antheridia. Only one egg develops per archegonium. o Why would male and female gametangia mature at different times on the same gametophyte? o What cellular division produces the gametes? o What is the ploidy level of the finished gametes? o Are the sperm flagellated or unflagellated? Make sure you know the answers to the following: 1) What characteristics limit the height of mosses and liverworts? 2) Given your answer to question 1, why is a moss blade so thin, almost see-through? 3) What features do ferns have that mosses and liverworts do not possess that make them better adapted for different environments & better dispersers of their spores? 4) What is the advantage of having sporangia above the gametophyte in mosses and liverworts? 5) You should be able to diagram the life cycle of a liverwort, moss, and fern. On your drawing you need to be able to label where meiosis & mitosis occur, what stages are unicellular or multicellular, and the ploidy level of each stage. Your drawings of the different plants should be recognizable as that plant type.
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