Dilutions Math in Excel

xlsx

School

Virginia Commonwealth University *

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Course

MISC

Subject

Biology

Date

Feb 20, 2024

Type

xlsx

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5

Uploaded by krisha753

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c1v1=c2v2 Find: v1 Given: v2 (in mL) 0.2 Dilution (D) D = 0.4 D = c2/c1 c1v1=c2v2 v1= (c2v2)/c1 v1 = (c2/c1) * v2 v1 = D * v2 0.08 80 mL microliters How many microliters of original sample are required to produce a final dilution of 10-1 in a total volume of 0.2 mL?
Given: Serial Dilution of 0.001 259 CFU on plate (from 0.1 mL of the indicated dilution) Find: Bacteria per mL of original culture Total Dilution (D) = dilution D = dilution * 0.1 mL 0.001 Diluted CFU/mL 259 CFU / mL original culture = CFU / mL * D^-1 2.59E+06 A 0.001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 259 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?
0.1mL total dilution 0.1 1.00E-04
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Given: original cell density 6.00E+09 CFU on plate (cfu/0.1mL) 30 Cell Density of CFU on plate 300 Find: Dilution, means we need to find V1 v2 (mL) 0.1 m1 (cells/mL) 6.00E+09 m1v1 = m2v2 v1 = (m2v2)/m1 5.00E-09 If you suspect your culture of bacteria has 60*10^8 cells per mL, how many mL of original culture would you want to end up on the plate to produce 30 cfu?
< typed in 60E8 but it automatically puts in that

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