Copy of Lab 2 Equipment Post-lab_FA22

pdf

School

University of California, Berkeley *

*We aren’t endorsed by this school

Course

3A

Subject

Biology

Date

Feb 20, 2024

Type

pdf

Pages

5

Uploaded by julianakim64

Report
Post-lab Assignment LAB 2: Equipment Use Bio 1AL Fall 2022 Last Name __________Kim______________ First Name ___________Juliana___________ Lab Section #_____223______ Lab 2 Post-Lab 1) Based upon your absorption spectrum of DCPIP, record your value for λ max . (0.5 pt) Λ max : 606.3 2) DCPIP standard curve a) Graph the quantitative relationship between absorbance and DCPIP concentration using the DCPIP standard curve absorbance values that you recorded. See the Correlation and Regression Analysis page in bCourses. Use an xy scatter plot chart type in Excel, and fit the data with a linear trendline. Show the equation of the trendline and R 2 value on the graph. Save the graph in Excel as an image ( Ctrl Click on the graph > Save as Picture or take a screenshot ) and paste the image below. (1 pt) X-axis: DCPIP Concentration ( µM) Y-axis: Absorbance (nm) b) Describe in your own words what your R 2 value means. (0.5 pt) Our R squared value came out to 0.9627 meaning that 96.27% of the variation in absorbance values is explained by the linear relationship with DCPIP values. 2-1
Post-lab Assignment LAB 2: Equipment Use Bio 1AL Fall 2022 c) Use your standard curve to determine the concentration (in µM) of the unknown DCPIP solution. Report the absorbance that you measured for the unknown DCPIP solution. Use the equation of the trendline for your calculation, and show your work. Include units. (0.5 pt) Absorbance of unknown DCPIP solution: ______0.834 nm_______ Concentration of unknown DCPIP solution: ____26.49 µM _____ (Show your work below). Y = 0.0255x - 0.1584 (0.834 nm) = 0.0255 x - 0.1584 0.6756 = 0.0255x X = 26.49 µM 3) Pipetting : Record your five values for pipetting 100 μL and those of your lab station partner. Note if you did not have a station partner then get the data from another student. If there were three students in your group, then please record the data of one of them. a) You will be running a t-test on your data. State the null hypothesis for the t-test. (0.5 pts) We hypothesize that the difference in the group means will be zero. b) Calculate the mean, standard deviation (see Excel Graphing Resources), and percent error from the expected weight for the 100 μL sample. Run an Independent Sample t-test using VassarStats, and record the relevant values below. See the Comparing Two Means page in bCourses for guidance on performing and interpreting the t-test. (2 pts) Your data Lab partner’s data Observed weights (g) 0.0976 0.0981 0.0999 0.0975 0.1 0.1002 0.0994 0.0989 0.1001 0.0999 Mean weight (g) 0.0994 0.09892 Standard deviation (g) .00104 0.00115 Percent error from expected (%) 0.6% 1.08% t-test results t = __0.69__, df = __7.92__, p (2-tailed) = __0.5086__ c) Based on the t-test results, what should you do with regard to the null hypothesis? (circle one) (0.5 pt) Reject the null hypothesis Fail to reject the null hypothesis 2-2
Post-lab Assignment LAB 2: Equipment Use Bio 1AL Fall 2022 4) In lecture, micropipette accuracy and precision were discussed. (0.5 pt) Which statistical measurement is most relevant and likely to be altered by errors arising from problems with: Pipettor accuracy ? (Circle/Highlight one): Mean Standard Deviation Pipettor precision ? (Circle/Highlight one): Mean Standard Deviation 5) Reticle calibration . Complete the table below using the data you recorded in Table 3 of the Lab 2 Procedures. (1.5 pt) Total Magnification # of Subdivisions in 100 μm Calibration (μm) = distance between each subdivision 100X # = ___10____ 100 μm / # = ___10___ μm 200X # = ___19___ 100 μm / # = __5.26__ μm 400X # = ___39____ 100 μm / # = _2.56_ μm 6) Depth of Field . Examine the slide with the solid glass rod and hollow glass tube. In the left circle, draw the appearance of a solid rod when the 20X objective is focused in the midpoint of the solid rod. In the right circle, draw the appearance of the hollow tube when focused in the midpoint of the hollow tube. Include scale bars labeled with the distance that they represent . (1 pt) Drawing instructions: If you hand-drew the drawings, you can take a picture of the drawings and insert the image below. You may also double click on the image below and complete the drawings using the Google Drawing tools. Which is on top : the solid rod or the hollow tube? (0.5 pt) (Circle/highlight one) Rod Tube 2-3
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
Post-lab Assignment LAB 2: Equipment Use Bio 1AL Fall 2022 7) Optical sections . As described in the Microscopy background reading, at high power objectives, the depth of field is very small. By repositioning the midpoint of the depth of field, you can generate different images (optical sections) of the specimen. Draw predicted images for each of the three focal points of the cell shown on the right side of Figure 8 of the Lab 2D - Using a Compound Microscope page in bCourses. The images should correspond to what you would see using the 40x objective. The circles below represent the field of view. Remember you are looking down on the cell, while the image in the lab manual shows a side view. (0.5 pt) Label the location of the cilia, cell membrane, nucleus, and cytoplasm in your drawings (Note: not all structures can be observed at each given focal point.) You do not need to include a scale bar in these drawings because one is not provided in Figure 8. (0.5 pt) Drawing instructions: If you hand-drew the drawings, you can take a picture of the drawings and insert the image below. You may also double click on the image below and complete the drawings using the Google Drawing tools. 2-4
Post-lab Assignment LAB 2: Equipment Use Bio 1AL Fall 2022 Post-lab continued on next page. 8) The artist David Hockney has proposed the controversial theory that the sharp emergence of more realistic paintings in the Renaissance came about because artists began using camera obscura devices to project images onto their canvases that they could then use as guides. One of the most interesting pieces of data to support his theory comes from quantitating the handedness of people in paintings before and after the emergence of realistic depictions. Hockney did this by looking at which hand people in paintings held objects like cups or canes, as shown in the Vermeer below, and he saw that prior to the emergence of realistic art, the vast majority of people were right-handed (the normal situation), and this shifted dramatically to mostly left-handed people (statistically unusual), in realistic paintings. Camera obscura devices alter images of what you are seeing in the same way as the light microscopes you are using in lab. Explain which of the slides you examined in lab supports Hockney’s theory that a microscope-like device was involved in creating the Vermeer above where the man is holding a cane with his left hand. (1 pt) I believe that the letter e slide is a perfect representation of the inversion that supports Hockney’s theory. In the lab, the letter is upside down and inverted, and left to right in reference to the specimen. In Hockney’s it makes sense that the art has mostly left-handed people which is statistically unusual, if artists did begin to use the camera obscura as guides because cameras often invert the image, left to right, or vice versa. 2-5

Related Documents

Doc2.docx
Exam 1.docx
Sterile Processing Technology, Part 1 28986 20231214140530.pdf
W01Quiz.docx
BIO 330 Discussion 2.docx
Copy of Lab 3 Post-lab_FA22.pdf
BIOL-2302-R.Q.2.pdf
BIOL-2302-R.Q.3.pdf
Bac:Gram Stain worksheet PDF.pdf
M1 Writing Assignment.docx
Bio 150 Test 3 ch 13-17.docx
Dilutions Math in Excel.xlsx
Recommended textbooks for you
Text book image
Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Text book image
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Text book image
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Text book image
Complete Textbook Of Phlebotomy
Biology
ISBN:9781337464314
Author:Hoeltke
Publisher:Cengage
Text book image
Medical Terminology for Health Professions, Spira...
Health & Nutrition
ISBN:9781305634350
Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:Cengage Learning
Text book image
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
SEE MORE TEXTBOOKS
Recommended textbooks for you
Text book image
Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Text book image
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Text book image
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Text book image
Complete Textbook Of Phlebotomy
Biology
ISBN:9781337464314
Author:Hoeltke
Publisher:Cengage
Text book image
Medical Terminology for Health Professions, Spira...
Health & Nutrition
ISBN:9781305634350
Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:Cengage Learning
Text book image
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
SEE MORE TEXTBOOKS