Lab Worksheet 4

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Feb 20, 2024

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BIO 1220L Lab 4 Worksheet: Protist “Lights, Camera, Theft?”: A Protist Whodunnit (Worksheet) Part 1—Setting the Stage 1. Think back…have you ever been swimming in a lake, river, stream, or pond and accidentally swallowed some water? Make a list of possible protists that you could have ingested. Some possible protists I could have come across are, slime molds and algae. 2. Fill in each row of the table with the results from your virtual pond dip experiment. Protist Name Habitat Mode of Locomotion Auto, Hetero, or Mixotrophic (Both)? Volvox Planktonic in still and flowing waters -whip-like tails (flagella) -undulating their flagella in unison, propelling the colony through the water. Mixotrophic Rotifers Some species are planktonic, others are attached to plants, other organisms, stones or creeping on plants, mud surface etc. -crown of hair-like cilia -locomote by using it to propel themselves. Heterotrophic Diatoms Planktonic, or attached to plants; often form brown crusts on surfaces under water. (stones, plant stems) -controlled secretions (glide) -move across surfaces via adhesive mucilage secreted through the raphe Autotrophic Amoeba Decaying organic matter e.g. on leaves and surface of bottom mud. -pseudopodia Heterotrophic Actinosphaeriu m Planktonic and amongst plants (especially fine-leaved) -pseudopodia Heterotrophic 3. Out of all the protists that you learned about, which ones were your favorite? Why? Give a detailed explanation. Out of all the protists that I learned about, the most intriguing was the amoeba. One of the things I found interesting is that their habitat is typically on decaying organic matter like the surface of bottom mud. I also found it interesting that they are unicellular protists that are able to change their shape constantly and locomote by ways of cytoplasmic movement. I learned that the amoeba forms pseudopods (false feet) with which they 'flow' over a surface and use to capture prey. This means that they have the ability to detect the kind of prey and have tactics to engulf it.

BIO 1220L | LAB WORKSHEET 4: PROTIST 4. What is an interesting fact that you learned about protists? An interesting fact that I learned about protists is that there are different types of protists such as those that are animal like, fungus like and plant like. I also learned that some protists can be deadly such as the Naegleria fowleri which is a “brain eating amoeba” found in warm freshwater. Part 2—Solving the Mystery While solving the clues provided to you in the Protists Activity, fill in this table to help you solve the mystery. You may do this electronically by clicking on the checkboxes and typing the motive in the respective column. The correct answer for each clue will either tell you one of the guest’s motives or their whereabouts when the crime occurred. Remember, the suspect must have a motive and must have been present at the scene of the crime . Guest List Motive Last Known Whereabouts ☐ Audrey Amoeba Audrey Amoeba was jealous of Ruby Rockweed because she did not get cast as the star actress in “Singin’ in the Pond ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Clyde “Dead Man’s Fingers” Codium Clyde “Dead Man’s Fingers” Codium was going secretly going bankrupt and needed something expensive to sell. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Danny Diatom Danny Diatom was fed up with all these actors and awards ceremonies ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Donny Dinoflagellate Donny Dinoflagellate is secretly in love with Audrey Amoeba and hated Ruby Rockweed for snubbing Audrey’s acting talents. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Eugena Euglena Eugena Euglena went to high school with Ruby Rockweed, who bullied her incessantly. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Katherine Kelp Katherine Kelp was nominated for the Protista Award that Ruby Rockweed won for her role in “My Fair Protist”, and she was upset ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Percy Plasmodium Percy Plasmodium was angry that his movie “My Fair Protist” did not win a Protista Award. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom

BIO 1220L | LAB WORKSHEET 4: PROTIST ☐ Petra Paramecium Petra Paramecium was best friends with Ruby Rockweed, and even believed that Ruby deserved the Protista win. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Philip Physarum As a wealthy socialite arising from “Old Money”, Philip Physarum hates all of these up and coming actors making money for terrible movies. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Rita Red Algae Rita Red Algae was the second pick for star actress in “Singin’ in the Pond”, but Ruby Rockweed got the role. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Ruby Rockweed Ruby Rockweed has always been a drama queen and loves to be the center of attention. Could have faked the whole crime? ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Sterling Spirogyra While Sterling Spirogyra may appear as a famous movie star, he has ties to a rival mob. They are looking for something big to steal and sell to make some money ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Tony Trichonympha Tony Trichonympha could care less about the Protista Awards. He was just at the party for free cocktails. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Ursula Ulva Word around town is that Ursula Ulva is a backstabber and was upset that she got snubbed in the Protista nominations. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom ☐ Vivien Volvox Vivien Volvox always dreamed of being an actress, but she was terrible at acting, so she hates movie stars. ☐ Billiard Room ☐ Cellar ☐ Parlor ☐ Ballroom Solve the Crime! (You may type directly in the text boxes below).

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BIO 1220L | LAB WORKSHEET 4: PROTIST Solving the Clues Record your answers to the clues provided to you in the Protists Activity . (Note—the possible answer choices are in the bolded column, i.e. the column talking about protists) 1. What structure(s) does the protozoan shown in the image use to move? (See image in activity)? -Pseudopodia 2. In the following image (see image in activity), where are these protozoans located? -Red Blood Cells 3. What is the primary function of the red eye spot in Euglena? -Detect light 4. What are the ribbon-shaped, helically-arranged structures that run the length of the filaments in Spirogyra? -Chloroplasts 5. What is the specific name of the process that Amoebas use to eat? -Phagocytosis 6. How do Paramecium move? -Cilia 7. Which of these protists have a contractile vacuole? -Euglena 8. What is the function of the contractile vacuole in Paramecium? -Water Balance 9. How does Volvox reproduce? -Both of the Above (sexually and asexually) 10. These organisms are the cause of a variety of biological phenomena, such as algal blooms. -Dinoflagellates 11. How do dinoflagellates move? -flagellum 12. Which organism is the largest of all of the protists? -Kelp 13. These organisms have two flagella, which gives them some control over mobility. -Dinoflagellates 14. An encounter with this protozoan can sometimes be deadly. -Amoeba

BIO 1220L | LAB WORKSHEET 4: PROTIST 15. Plasmodium uses these organisms to transfer itself from host to host. -Mosquitoes 16. These are the only organisms on the planet to have a cell wall made of glass. -Diatoms 17. Trichonympha is typically found in this environment. -Termite Gut 18. Protists that produce their food through photosynthesis are called what? -Autotrophs 19. Naegleria Fowleri can cause this devastating infection. -PAM 20. Protists were the cause of this event. -Irish Potato Famine 21. This organism is used as “nori” for seaweed wrappers. -Red Algae 22. This organism can be used to make salads, hence its nickname. -Ulva 23. Which organism is found in intertidal zones during low tide? -Rockweed 24. This green algae is a multicellular organism. -Cladophora 25. You may not want to come across this organism just based off of its nickname . -Codium 26. This protist is a colonial organism. -Volvox 27. Some of the protists that you learned about are: - All the Above 28. These organisms have no cell walls. -Protozoans 29. These organisms could potentially be used for biofuels and as a food source due to their fast reproductive rate. -Green Algae 30. These protists can be found as an abrasive in toothpaste! -Diatoms Part 3—Reflection Answer the following questions. Give detailed explanations. 1. A protist can be an autotroph, heterotroph, or potentially both. How is an autotroph different from a heterotroph? Autotrophs are organisms which produce their own food. They can make food from inorganic substances, such as water and carbon dioxide. Heterotrophs are organisms which rely on producers to get food. They can eat plants or other animals to get their energy. 2. Many protists have structures that allow them to move. What are some of these structures? The structures that allow for protists to move are Cilia, flagella or pseudopods.

BIO 1220L | LAB WORKSHEET 4: PROTIST 3. Compare and contrast the different types of protists. Animal like Protists- no cell walls, heterotrophic Fungus-like Protists- do have cell walls, heterotrophic Plant-like Protists- do have cell walls, autotrophic 4. Protists can be helpful to other organisms by… -Some protists decompose and recycle nutrients -Protists such as Diatoms produce carbohydrates and oxygen that other organisms can use. -Protists (Green algae) play a major role as producers because they provide food for aquatic organisms -Some green algae have a rapid reproductive rate and scientist have proposed using these green algae for biofuels and food for humans and livestock -Protists such as Dinoflagellates produce oxygen, help corals remove waste and give the coral glucose, glycerol and amino acids 5. Protists can be harmful to other organisms by… -some protists are parasites and encounters with them can be fatal -plasmodium=malaria -brain eating amoeba=PAM

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