Project Proposal TJagjyot Kaur and Muhhamed Sani

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Centennial College *

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CH303

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Biology

Date

Jun 7, 2024

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docx

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7

Uploaded by SuperHumanFlamingo4372

PROJECT PROPOSAL Microbiology Project 3 (BI 303) Instructions to use the template: Fill in the boxes/ tables/ chats with precise details from your projects. Do not change the format (font size, spacing) nor box dimension. Some brief examples from various hypothetical projects are provided and you are supposed to replace those with your own. Please note that theses examples are not complete, you’ll get better guidance from your professors. Group Members: Jagjyot Kaur Muhammed Sani 1. TITLE: Constructing an Antibacterial Loofah using Nanoparticle technology 2. RATIONALE: (Importance of the topic & why you are doing this) 3. AIM/ MAJOR OBJECTIVES: (in point form) Constructing an antibacterial Loofah using Nanoparticle technology to test the antibacterial efficacy of active ingredients Cinnamaldehyde and thymol and compare it to commercially available natural and synthetic loofah Developing and evaluating an antibacterial loofah using nanoparticle technology with cinnamaldehyde and thymol is crucial for improving hygiene by reducing bacterial growth and lowering skin infection risks. Since many studies have shown that loofah can promote growth of microorganism like Pseudomonas aeruginosa , Serratia marcescens , Escherichia coli, Bacillus species , Enterococcus species without additional nutritional supply. This approach leverages natural antibacterial components to meet market demand for safe, effective products and ensures continuous delivery through nanotechnology. Offers a competitive advantage in the personal care industry and promoting better health and well-being (Bottone et al., 1994). (Maximum 200 words) 1.Preparing Cinnamaldehyde and thymol Nanoparticles 2.Confirm whether the nanoparticles have antibacterial property using well diffusion method 3.Comparing Nanoparticle coated Loofah to commercially available loofah
4. RESEARCH DESIGN Major Activities/ Experiments Hypotheses Iterations Literature survey, Lab inventories, purchase order/ raw material purchase Sample collection (if applicable), common media prep, culture maintenance. Qualitative research (surveys, data analysis) Preparatory phase (Week 1 – 4) Preparing Solution Performing Nano herbal encapsulation Confirming encapsulation using spectrophotometry method Iteration 1: Preparing Cinnamaldehyde and thymol Nanoparticles Hypothesis 1: Nano- herbal encapsulation of Cinnamaldehyde and thymol carried out successfully Using well diffusion assay to check antibacterial efficacy of prepared nanoparticles against Pseudomonas aeruginosa and Bacillus subtilis Spraying the loofah with nanoparticles Performing parallel Streak method Iteration 2: Checking Antibacterial efficacy of prepared nanoparticle Hypothesis 2: Zone of clearance was observed for well diffusion assay. Nanoparticle sprayed loofah shows antibacterial effect against test organism Iteration 3: Comparing Nanoparticle coated Loofah to commercially available loofah Hypothesis 3: Nanoparticle coat loofah showed reduction in bacterial growth than commercially available loofah Spiking loofahs with test organism Air drying at different intervals Centrifuging to obtained suspension Serial dilution and spread plating
5. METHOD a. Supplies: Organisms Media Major equipment Other supplies (e.g., kits, raw material) 1 Bacillus subtilis TSA Spectrophotometer Pentasodium tripolyphosphate (TPP) 2 Pseudomonas aerogenes Phosphate- buffered saline (PBS Incubator Sterile spreaders 3 TSB Sterile forceps and tweezers 4 MacConkey Agar Shaker Sterile pipettes and pipette tips 5 Centrifuge Mortar and pestle 6 Filter paper 7 Petri plates 8 Buffer solutions 9 Eppendorf tubes /Centrifuge tubes 10 Stainless steel cylinders 11 Tweezers 12 Chitosin, Tween80 13 Cinnamaldehyde, thymol Sterile tubes b. Choice of Methods/ Technique using a Decision Matrix Please refer to the guide (pdf) and use the template (Excel) to build your own matrix. Make sure you fill up both the “ranking” and “justification” tabs and upload as “Excel file”.
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