Gram + organisms *Colony color refers to growth on nutrient agar at room temperature. White, cream, yellow, and 'yellowish' are very difficult to distinguish from one another and should not be used to ID bacteria. However, gold and rose are very distinctive and therefore can and should be used to confirm identification! REVISED FEB. 2018 Organism Bacillus subtilis + Bacillus cereus Staphylococcus aureus Gram Shape Staphylococcus epidermidis + Bac Cream Bac + Coc Colony color + Coc Cream Yellow- ish Cream/ white Mannitol Maltose Acid Acid Acid Fermentation Galactose Acid Acid Lactose Glucose Acid Acid Acid Acid Acid Acid Gelat- Cata- inase lase + + + +/- Amy- lase + ' Urease + Methyl red + + + Nitrate reduc- tase + +

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 24RQ
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Create a dichotomous key please
Gram + organisms
*Colony color refers to growth on nutrient agar at room temperature. White, cream, yellow, and 'yellowish' are very difficult to
distinguish from one another and should not be used to ID bacteria. However, gold and rose are very distinctive and therefore can
and should be used to confirm identification!
REVISED FEB. 2018
Colony
Fermentation
Nitrate
Gelat-
Cata- Amy-
Methyl
Organism
Gram Shape
color
Urease
reduc-
inase
lase
lase
red
Mannitol
Maltose
Galactose
Lactose
Glucose
tase
Bacillus subtilis
Вас
Cream
Acid
Bacillus cereus
Вас
Cream
Acid
Acid
Staphylococcus
Yellow-
Сос
Acid
Acid
Acid
Acid
Acid
aureus
ish
Cream/
Staphylococcus
epidermidis
Сос
Acid
Acid
Acid
+/-
white
Enterococcus
Сос Cream
Acid
Acid
Acid
Acid
Acid
faecalis
Sarcina
Сос
Gold
aurantiaca
Sarcina lutea
Coc Yellow
Micrococcus
Сос
Rose
roseus
-/+
Cream/
Streptococcus
salivarius
+
Сос
Acid
Acid
Acid
Acid
white
Sporosarcina
Yellow-
Сос
+/-
ureae
ish
+
+
+
+
+ +
+
+ + +
+
Transcribed Image Text:Gram + organisms *Colony color refers to growth on nutrient agar at room temperature. White, cream, yellow, and 'yellowish' are very difficult to distinguish from one another and should not be used to ID bacteria. However, gold and rose are very distinctive and therefore can and should be used to confirm identification! REVISED FEB. 2018 Colony Fermentation Nitrate Gelat- Cata- Amy- Methyl Organism Gram Shape color Urease reduc- inase lase lase red Mannitol Maltose Galactose Lactose Glucose tase Bacillus subtilis Вас Cream Acid Bacillus cereus Вас Cream Acid Acid Staphylococcus Yellow- Сос Acid Acid Acid Acid Acid aureus ish Cream/ Staphylococcus epidermidis Сос Acid Acid Acid +/- white Enterococcus Сос Cream Acid Acid Acid Acid Acid faecalis Sarcina Сос Gold aurantiaca Sarcina lutea Coc Yellow Micrococcus Сос Rose roseus -/+ Cream/ Streptococcus salivarius + Сос Acid Acid Acid Acid white Sporosarcina Yellow- Сос +/- ureae ish + + + + + + + + + + +
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