What are the chemicals and materials used in gel electrophoresis?
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Gel electrophoresis
Questions
1. What are the chemicals and materials used in gel electrophoresis?
2. Draw a schematic diagram of a gel electrophoresis set-up
3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?
4. What is the use of ethidium bromide and why must you wear gloves when you handle it?
5. What makes the DNA fragment move towards the positive plate?
6. What is the purpose of glycerol in the sample buffer?
7. What is the use of a DNA ladder?
8. What will happen when you increase the voltage of the set-up?
9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
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