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2. In order to make CDNA, RNA needs to be isolate. Describe in detail the steps for the isolation of total RNA from the cells using the TRI-Reagant method. thod

2. In order to make CDNA, RNA needs to be isolate. Describe in detail the steps for the isolation of total RNA from the cells using the TRI-Reagant method. thod

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Help me with question 2 please.
References Mailings out Review View Help 1. Tissue culture is a term that refers to both organ culture and cell culture and the terms are often used interchangeably. Cell cultures are derived from either primary tissue explants or cell suspensions. Primary cell cultures typically will have a finite life span in culture whereas continuous cell lines are, by definition, abnormal and are often transformed cell lines. 2. In order to make cDNA, RNA needs to be isolate. Describe in detail the steps for the isolation of total RNA from the cells using the TRI-Reagant method. 3. Describe in detail the PCR method 4. Describe five items found in cell media that are required for the growth and maintenance of cells in culture 5. Describe the different components of the image Analysis system 6. D. Focus 38°F 口 F11 F10
Transcribed Image Text:References Mailings out Review View Help 1. Tissue culture is a term that refers to both organ culture and cell culture and the terms are often used interchangeably. Cell cultures are derived from either primary tissue explants or cell suspensions. Primary cell cultures typically will have a finite life span in culture whereas continuous cell lines are, by definition, abnormal and are often transformed cell lines. 2. In order to make cDNA, RNA needs to be isolate. Describe in detail the steps for the isolation of total RNA from the cells using the TRI-Reagant method. 3. Describe in detail the PCR method 4. Describe five items found in cell media that are required for the growth and maintenance of cells in culture 5. Describe the different components of the image Analysis system 6. D. Focus 38°F 口 F11 F10
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