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Assignment 2B: Presented below are the results you obtained from the abovementioned experiment. Determine the total quantity of bacteria present in the sample sponge/dishcloth (as CFU/ml). What are the implications of your results? Results obtained: Number of colonies isolated from 10 kitchen sponges Sponge Plate 2 ABCDEFGH_- J Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 3 Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 4 389 511 908 412 575 602 425 376 523 605 Plate 5 98 53 294 118 263 209 225 154 274 242 Plate 6 2 26 29 25 23 21 5 11 18 22

Assignment 2B: Presented below are the results you obtained from the abovementioned experiment. Determine the total quantity of bacteria present in the sample sponge/dishcloth (as CFU/ml). What are the implications of your results? Results obtained: Number of colonies isolated from 10 kitchen sponges Sponge Plate 2 ABCDEFGH_- J Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 3 Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 4 389 511 908 412 575 602 425 376 523 605 Plate 5 98 53 294 118 263 209 225 154 274 242 Plate 6 2 26 29 25 23 21 5 11 18 22

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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This is a microbiology question. please help.

Materials and methods ● ● ● Bring a sponge or dishcloth used in the kitchen from home/residence. Cut the sponge/dishcloth into sections no bigger than 10cm². Submerge the sponge/dishcloth in a plastic bag containing 50 ml peptone broth to extract bacteria from the item. Seal the bag. Knead the sponge/dishcloth in the broth for 3 minutes. With sterile forceps remove the sponge/dishcloth and place the item into a sterile plastic bag. Seal the bag. Wring the liquid from the sponge or dishcloth (in the bag) using your hands. Dilute the extract and plate onto an LB and MacConkey agar plate (dilution plating method, refer to section below). After incubation at 25°C for 7 days, count the number of bacterial colonies and determine the CFU/ml. Dilution plate method: Take six Eppendorf tubes and pipette exactly 900 µl of Ringer's solution into each. Label the tubes clearly from 10-¹ to 10-6. Pipette 100 μl of the extract into tube no 1 (10-1 dilution). Mix well. Pipette 100 μl of the 10-1 dilution into the next tube and mix (10-2 dilution). Repeat up to the 10-6 dilution. Label four petri dishes 10-2, 10³, 104 and 10-5. Now pipette 100 μl of each dilution into the corresponding petri dish. Using a sterile glass rod spread the bacterial suspension over the surface of the agar.
Transcribed Image Text:Materials and methods ● ● ● Bring a sponge or dishcloth used in the kitchen from home/residence. Cut the sponge/dishcloth into sections no bigger than 10cm². Submerge the sponge/dishcloth in a plastic bag containing 50 ml peptone broth to extract bacteria from the item. Seal the bag. Knead the sponge/dishcloth in the broth for 3 minutes. With sterile forceps remove the sponge/dishcloth and place the item into a sterile plastic bag. Seal the bag. Wring the liquid from the sponge or dishcloth (in the bag) using your hands. Dilute the extract and plate onto an LB and MacConkey agar plate (dilution plating method, refer to section below). After incubation at 25°C for 7 days, count the number of bacterial colonies and determine the CFU/ml. Dilution plate method: Take six Eppendorf tubes and pipette exactly 900 µl of Ringer's solution into each. Label the tubes clearly from 10-¹ to 10-6. Pipette 100 μl of the extract into tube no 1 (10-1 dilution). Mix well. Pipette 100 μl of the 10-1 dilution into the next tube and mix (10-2 dilution). Repeat up to the 10-6 dilution. Label four petri dishes 10-2, 10³, 104 and 10-5. Now pipette 100 μl of each dilution into the corresponding petri dish. Using a sterile glass rod spread the bacterial suspension over the surface of the agar.
Assignment 2B: Presented below are the results you obtained from the abovementioned experiment. Determine the total quantity of bacteria present in the sample sponge/dishcloth (as CFU/ml). What are the implications of your results? Results obtained: Number of colonies isolated from 10 kitchen sponges Sponge Plate 2 ABCDEFGHL J Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 3 Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 4 389 511 908 412 575 602 425 376 523 605 Plate 5 98 53 294 118 263 209 225 154 274 242 Plate 6 2 26 29 25 23 21 5 11 18 22
Transcribed Image Text:Assignment 2B: Presented below are the results you obtained from the abovementioned experiment. Determine the total quantity of bacteria present in the sample sponge/dishcloth (as CFU/ml). What are the implications of your results? Results obtained: Number of colonies isolated from 10 kitchen sponges Sponge Plate 2 ABCDEFGHL J Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 3 Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Too many to count Plate 4 389 511 908 412 575 602 425 376 523 605 Plate 5 98 53 294 118 263 209 225 154 274 242 Plate 6 2 26 29 25 23 21 5 11 18 22
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