Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 9.4, Problem 15BYGO
Summary Introduction
To write:
The major problems engineers face while designing joint prostheses and its common cause of failure.
Introduction:
The joint prosthesis is the device that is used as a substitute to replace diseased bones. The treatment is known as Arthroplasty.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 9 Solutions
Human Anatomy
Ch. 9.1 - Prob. 1AWYKCh. 9.1 - Prob. 1BYGOCh. 9.1 - Prob. 2BYGOCh. 9.1 - Give some examples of joints that become...Ch. 9.1 - Prob. 4BYGOCh. 9.1 - Prob. 5BYGOCh. 9.1 - Prob. 6BYGOCh. 9.2 - Prob. 1AWYKCh. 9.2 - Prob. 2AWYKCh. 9.2 - What are the two components of a joint capsule?...
Ch. 9.2 - Give at least one example each of a monaxial,...Ch. 9.2 - Prob. 9BYGOCh. 9.3 - Where else in the body is there a structure...Ch. 9.3 - Prob. 10BYGOCh. 9.3 - Prob. 11BYGOCh. 9.3 - What keeps the femur from slipping backward off...Ch. 9.3 - Prob. 13BYGOCh. 9.4 - Define arthritis. How do the causes of...Ch. 9.4 - Prob. 15BYGOCh. 9 - Prob. 9.1.1AYLOCh. 9 - Prob. 9.1.2AYLOCh. 9 - Prob. 9.1.3AYLOCh. 9 - Prob. 9.1.4AYLOCh. 9 - Prob. 9.1.5AYLOCh. 9 - Prob. 9.1.6AYLOCh. 9 - Prob. 9.1.7AYLOCh. 9 - Prob. 9.2.1AYLOCh. 9 - Prob. 9.2.2AYLOCh. 9 - Prob. 9.2.3AYLOCh. 9 - Prob. 9.2.4AYLOCh. 9 - Prob. 9.2.5AYLOCh. 9 - Prob. 9.2.6AYLOCh. 9 - Prob. 9.2.7AYLOCh. 9 - Prob. 9.2.8AYLOCh. 9 - The same for elevation and drpessionCh. 9 - Prob. 9.2.10AYLOCh. 9 - Prob. 9.2.11AYLOCh. 9 - Prob. 9.2.12AYLOCh. 9 - Prob. 9.2.13AYLOCh. 9 - Prob. 9.2.14AYLOCh. 9 - The same for rotation of the head or torsoCh. 9 - The same for lateral and medial excursion of the...Ch. 9 - Prob. 9.2.17AYLOCh. 9 - Prob. 9.2.18AYLOCh. 9 - The same for the ankle or foot movements of...Ch. 9 - Prob. 9.2.20AYLOCh. 9 - Prob. 9.3.1AYLOCh. 9 - Prob. 9.3.2AYLOCh. 9 - Prob. 9.3.3AYLOCh. 9 - Prob. 9.3.4AYLOCh. 9 - Special functional qualities of the tibiofemoral...Ch. 9 - Prob. 9.3.6AYLOCh. 9 - The range of discorders included in the concept of...Ch. 9 - The general meaning of arthritis, and the...Ch. 9 - Prob. 9.4.3AYLOCh. 9 - Prob. 1TYRCh. 9 - Which of the following is the least movable? a...Ch. 9 - Prob. 3TYRCh. 9 - Prob. 4TYRCh. 9 - Prob. 5TYRCh. 9 - Prob. 6TYRCh. 9 - Prob. 7TYRCh. 9 - Prob. 8TYRCh. 9 - To bend backward at the waist involves ________ of...Ch. 9 - Prob. 10TYRCh. 9 - Th lubricant of a diarthrosis is _________.Ch. 9 - Prob. 12TYRCh. 9 - Prob. 13TYRCh. 9 - Prob. 14TYRCh. 9 - Prob. 15TYRCh. 9 - Prob. 16TYRCh. 9 - Prob. 17TYRCh. 9 - Prob. 18TYRCh. 9 - Prob. 19TYRCh. 9 - Prob. 20TYRCh. 9 - Prob. 1BYMVCh. 9 - Prob. 2BYMVCh. 9 - Prob. 3BYMVCh. 9 - Prob. 4BYMVCh. 9 - Prob. 5BYMVCh. 9 - Prob. 6BYMVCh. 9 - Prob. 7BYMVCh. 9 - Prob. 8BYMVCh. 9 - Prob. 9BYMVCh. 9 - State a meaning of each word element and give a...Ch. 9 - Prob. 1TOFCh. 9 - Prob. 2TOFCh. 9 - Prob. 3TOFCh. 9 - Determine which five of the following statements...Ch. 9 - Prob. 5TOFCh. 9 - Prob. 6TOFCh. 9 - Prob. 7TOFCh. 9 - Prob. 8TOFCh. 9 - Prob. 9TOFCh. 9 - Prob. 10TOFCh. 9 - Prob. 1TYCCh. 9 - Prob. 2TYCCh. 9 - In order of occurrence, list the joint actions...Ch. 9 - Prob. 4TYCCh. 9 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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