Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 9, Problem 2TYC
Summary Introduction
To write:
The name of ligament that breaks during the condition specified and the name of this condition.
Introduction:
Ankle joint is also known as talocrural joint and has two articulations, namely, lateral joint (between talus and fibula) and medial joint (between talus and tibia).
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 9 Solutions
Human Anatomy
Ch. 9.1 - Prob. 1AWYKCh. 9.1 - Prob. 1BYGOCh. 9.1 - Prob. 2BYGOCh. 9.1 - Give some examples of joints that become...Ch. 9.1 - Prob. 4BYGOCh. 9.1 - Prob. 5BYGOCh. 9.1 - Prob. 6BYGOCh. 9.2 - Prob. 1AWYKCh. 9.2 - Prob. 2AWYKCh. 9.2 - What are the two components of a joint capsule?...
Ch. 9.2 - Give at least one example each of a monaxial,...Ch. 9.2 - Prob. 9BYGOCh. 9.3 - Where else in the body is there a structure...Ch. 9.3 - Prob. 10BYGOCh. 9.3 - Prob. 11BYGOCh. 9.3 - What keeps the femur from slipping backward off...Ch. 9.3 - Prob. 13BYGOCh. 9.4 - Define arthritis. How do the causes of...Ch. 9.4 - Prob. 15BYGOCh. 9 - Prob. 9.1.1AYLOCh. 9 - Prob. 9.1.2AYLOCh. 9 - Prob. 9.1.3AYLOCh. 9 - Prob. 9.1.4AYLOCh. 9 - Prob. 9.1.5AYLOCh. 9 - Prob. 9.1.6AYLOCh. 9 - Prob. 9.1.7AYLOCh. 9 - Prob. 9.2.1AYLOCh. 9 - Prob. 9.2.2AYLOCh. 9 - Prob. 9.2.3AYLOCh. 9 - Prob. 9.2.4AYLOCh. 9 - Prob. 9.2.5AYLOCh. 9 - Prob. 9.2.6AYLOCh. 9 - Prob. 9.2.7AYLOCh. 9 - Prob. 9.2.8AYLOCh. 9 - The same for elevation and drpessionCh. 9 - Prob. 9.2.10AYLOCh. 9 - Prob. 9.2.11AYLOCh. 9 - Prob. 9.2.12AYLOCh. 9 - Prob. 9.2.13AYLOCh. 9 - Prob. 9.2.14AYLOCh. 9 - The same for rotation of the head or torsoCh. 9 - The same for lateral and medial excursion of the...Ch. 9 - Prob. 9.2.17AYLOCh. 9 - Prob. 9.2.18AYLOCh. 9 - The same for the ankle or foot movements of...Ch. 9 - Prob. 9.2.20AYLOCh. 9 - Prob. 9.3.1AYLOCh. 9 - Prob. 9.3.2AYLOCh. 9 - Prob. 9.3.3AYLOCh. 9 - Prob. 9.3.4AYLOCh. 9 - Special functional qualities of the tibiofemoral...Ch. 9 - Prob. 9.3.6AYLOCh. 9 - The range of discorders included in the concept of...Ch. 9 - The general meaning of arthritis, and the...Ch. 9 - Prob. 9.4.3AYLOCh. 9 - Prob. 1TYRCh. 9 - Which of the following is the least movable? a...Ch. 9 - Prob. 3TYRCh. 9 - Prob. 4TYRCh. 9 - Prob. 5TYRCh. 9 - Prob. 6TYRCh. 9 - Prob. 7TYRCh. 9 - Prob. 8TYRCh. 9 - To bend backward at the waist involves ________ of...Ch. 9 - Prob. 10TYRCh. 9 - Th lubricant of a diarthrosis is _________.Ch. 9 - Prob. 12TYRCh. 9 - Prob. 13TYRCh. 9 - Prob. 14TYRCh. 9 - Prob. 15TYRCh. 9 - Prob. 16TYRCh. 9 - Prob. 17TYRCh. 9 - Prob. 18TYRCh. 9 - Prob. 19TYRCh. 9 - Prob. 20TYRCh. 9 - Prob. 1BYMVCh. 9 - Prob. 2BYMVCh. 9 - Prob. 3BYMVCh. 9 - Prob. 4BYMVCh. 9 - Prob. 5BYMVCh. 9 - Prob. 6BYMVCh. 9 - Prob. 7BYMVCh. 9 - Prob. 8BYMVCh. 9 - Prob. 9BYMVCh. 9 - State a meaning of each word element and give a...Ch. 9 - Prob. 1TOFCh. 9 - Prob. 2TOFCh. 9 - Prob. 3TOFCh. 9 - Determine which five of the following statements...Ch. 9 - Prob. 5TOFCh. 9 - Prob. 6TOFCh. 9 - Prob. 7TOFCh. 9 - Prob. 8TOFCh. 9 - Prob. 9TOFCh. 9 - Prob. 10TOFCh. 9 - Prob. 1TYCCh. 9 - Prob. 2TYCCh. 9 - In order of occurrence, list the joint actions...Ch. 9 - Prob. 4TYCCh. 9 - Prob. 5TYC
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