EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 5SA
Why are Gram-negative bacteria more susceptible to heat than Gram-positive bacteria?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 9 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 9 - Why does milk eventually go bad despite being...Ch. 9 - Prob. 1EDCSCh. 9 - Why are BSL-4 suits pressurized? Why not just wear...Ch. 9 - Prob. 3TMWCh. 9 - Prob. 4TMWCh. 9 - Prob. 1MCCh. 9 - Prob. 2MCCh. 9 - Prob. 3MCCh. 9 - Prob. 4MCCh. 9 - Prob. 5MC
Ch. 9 - Prob. 6MCCh. 9 - Prob. 7MCCh. 9 - Prob. 8MCCh. 9 - The presarva1icn of beef jerky from microbial...Ch. 9 - Prob. 10MCCh. 9 - Which of the following substances would most...Ch. 9 - Which of the following adjectives best describes a...Ch. 9 - Prob. 13MCCh. 9 - Prob. 14MCCh. 9 - Prob. 15MCCh. 9 - Prob. 16MCCh. 9 - Which of the following disinfectants acts against...Ch. 9 - Which of the following disinfectants contains...Ch. 9 - Prob. 19MCCh. 9 - Prob. 20MCCh. 9 - Describe three types of microbes that are...Ch. 9 - Compare and contrast four tests that have been...Ch. 9 - Prob. 3SACh. 9 - Why do warm disinfectant chemicals generally work...Ch. 9 - Why are Gram-negative bacteria more susceptible to...Ch. 9 - Describe five physical methods of microbial...Ch. 9 - Prob. 7SACh. 9 - Prob. 8SACh. 9 - Compare and contrast desiccation and...Ch. 9 - Prob. 10SACh. 9 - Prob. 11SACh. 9 - Prob. 12SACh. 9 - Prob. 13SACh. 9 - What are some advantages and disadvantages of...Ch. 9 - Prob. 15SACh. 9 - Calculate the decimal reduction time (D) for the...Ch. 9 - Prob. 2VICh. 9 - Prob. 1CTCh. 9 - Prob. 2CTCh. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Over 1000 people developed severe diarrhea, and at...Ch. 9 - Prob. 6CTCh. 9 - Prob. 7CTCh. 9 - Prob. 8CTCh. 9 - Prob. 9CTCh. 9 - Prob. 10CTCh. 9 - Prob. 11CTCh. 9 - Where should you place a sterilization indicator...Ch. 9 - Why is liquid water necessary for microbial...Ch. 9 - Prob. 14CTCh. 9 - During the fall 2001 bioterrorist attack in which...Ch. 9 - What common household antiseptic contains a heavy...Ch. 9 - What is the phenol coefficient of phenol when used...Ch. 9 - Prob. 1CM
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