Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 9, Problem 2P
For each of the restriction enzymes listed below: (i) Approximately how many restriction fragments would result from digestion of the human genome (3 × 109 bases) with the enzyme? (ii) Estimate the average size of the pieces of the human genome produced by digestion with the enzyme. (iii) State whether the fragments of human DNA produced by digestion with the given restriction enzyme would have sticky ends with a 5′ overhang, sticky ends with a 3′ overhang, or blunt ends. (iv) If the enzyme produces sticky ends, would all the overhangs on all the ends produced on all fragments of the human genome with that enzyme be identical, or not? (The recognition sequence on one strand for each enzyme is given in parentheses, with the 5′ end written at the left. N means any of the four
| a. | Sau3A (^GATC) |
| b. | BamHI (G^GATCC) |
| c. | HpaII (C^CGG) |
| d. | SphI (GCATG^C) |
| e. | NaeI (GCC^GGC) |
| f. | BanI (G^GYRCC) |
| g. | BstYI (R^GATCY) |
| h. | BslI (CCNNNNN^NNGG) |
| i. | SbfI (CCTGCA^GG) |
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walnut
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Chapter 9 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Prob. 5PCh. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - Prob. 8PCh. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...
Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Prob. 12PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - Prob. 16PCh. 9 - Prob. 17PCh. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - showed part of the sequence of the plasmid vector...Ch. 9 - List three independent techniques you could use to...Ch. 9 - Figure 9.11a on p. 318 has numbers indicating the...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - Prob. 23PCh. 9 - a. What sequence information about a gene is...Ch. 9 - Why do geneticists studying eukaryotic organisms...Ch. 9 - Prob. 26PCh. 9 - The human genome has been sequenced, but we still...Ch. 9 - This problem investigates issues encountered in...Ch. 9 - Give two different reasons for the much higher...Ch. 9 - Using a cDNA library, you isolated two different...Ch. 9 - Prob. 31PCh. 9 - An interesting phenomenon found in vertebrate DNA...Ch. 9 - Prob. 33PCh. 9 - Prob. 34PCh. 9 - In the human immune system, so-called B cells can...Ch. 9 - Chimpanzees have a set of hemoglobin genes very...Ch. 9 - Complete genome sequences indicate that the human...Ch. 9 - On your computers browser, view the page accessed...Ch. 9 - Prob. 39PCh. 9 - Certain individuals with mild forms of...Ch. 9 - The 1 and 2 genes in humans are identical in their...
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