Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 9, Problem 18P
| a. | To make a genomic library useful for sequencing an entire genome, why would you ordinarily fragment the genomic DNA by mechanical shearing forces like sonication rather than by cutting the DNA with a restriction enzyme? |
| b. | Suppose that you wanted to make a genomic library to determine the complete sequence of a newly discovered organism’s genome, but you did not have a sonicator readily available. Explain how you could nonetheless use two or more restriction enzymes to make libraries whose clones could be sequenced so that a computer could assemble the genomic sequence. |
| c. | Suppose you only had a single restriction enzyme available, and you want to make a single genomic library from which you could assemble the genomic sequence. How might you be able to achieve this goal? (Hint: See Problem 7.) To make this library, would it be preferable to use a restriction enzyme that recognizes a 4-base, 6-base, or 8-base sequence of DNA? |
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Chapter 9 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Prob. 5PCh. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - Prob. 8PCh. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...
Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Prob. 12PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - Prob. 16PCh. 9 - Prob. 17PCh. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - showed part of the sequence of the plasmid vector...Ch. 9 - List three independent techniques you could use to...Ch. 9 - Figure 9.11a on p. 318 has numbers indicating the...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - Prob. 23PCh. 9 - a. What sequence information about a gene is...Ch. 9 - Why do geneticists studying eukaryotic organisms...Ch. 9 - Prob. 26PCh. 9 - The human genome has been sequenced, but we still...Ch. 9 - This problem investigates issues encountered in...Ch. 9 - Give two different reasons for the much higher...Ch. 9 - Using a cDNA library, you isolated two different...Ch. 9 - Prob. 31PCh. 9 - An interesting phenomenon found in vertebrate DNA...Ch. 9 - Prob. 33PCh. 9 - Prob. 34PCh. 9 - In the human immune system, so-called B cells can...Ch. 9 - Chimpanzees have a set of hemoglobin genes very...Ch. 9 - Complete genome sequences indicate that the human...Ch. 9 - On your computers browser, view the page accessed...Ch. 9 - Prob. 39PCh. 9 - Certain individuals with mild forms of...Ch. 9 - The 1 and 2 genes in humans are identical in their...
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