Concept explainers
(a)
To describe: The labeling pattern of glycophorin A, when a membrane-impermeable protein labeling reagent is added to a preparation of solubilized erythrocyte proteins.
Concept introduction: Glycophorin is a sialoglycoprotein in the red blood cell membrane. Glycophorins are rich in sialic acid and it carries sugar molecules. It is a membrane spanning protein which is heavily glycosylated. The presence of sialic acid gives the red blood cell a hydrophilic charged coat. Glycophorin A also known as GYPA belongs to the family of glycophorins.
(b)
To describe: The labeling pattern of glycophorin A when a membrane-impermeable protein labeling reagent is added to the intact erythrocyte ghosts.
Concept introduction: Glycophorin is a sialoglycoprotein in the red blood cell membrane. Glycophorins are rich in the sialic acid and it carries sugar molecules. It is a membrane spanning protein which is heavily glycosylated. The presence of sialic acid gives the red blood cell a hydrophilic charged coat. Glycophorin A also known as GYPA belongs to the family of glycophorins.
(c)
To describe: The labeling pattern of glycophorin A, when a membrane-impermeable protein labeling reagent is added to an erythrocyte ghosts that are initially leaky and then immediately sealed and transferred to a solution that does not contain the labeling reagent.
Concept introduction: Glycophorin is a sialoglycoprotein in the red blood cell membrane. Glycophorins are rich in the sialic acid and it carries sugar molecules. It is a membrane spanning protein which is heavily glycosylated. The presence of sialic acid gives the red blood cell a hydrophilic charged coat. Glycophorin A also known as GYPA belongs to the family of glycophorins.
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