FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
5th Edition
ISBN: 9781119498742
Author: Voet
Publisher: WILEY
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Chapter 9, Problem 11E
Summary Introduction
To explain: The reason why cultured animal cells can survive in the absence of linoleic acid.
Concept introduction: Linoleic acid is a type of essential fatty acid. It is needed for human growth and development. It is a polyunsaturated omega-6 fatty acid having an 18-carbon chain with two double bonds in the cis configuration. It occurs in nature as a triglyceride ester. It helps the body to increase the
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 9 Solutions
FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
Ch. 9 - Prob. 1ECh. 9 - 2. Which triacylglycerol yields more energy on...Ch. 9 - 3. Draw the structure of a glycerophospholipid...Ch. 9 - 4. Name this lipid.
Ch. 9 - Prob. 5ECh. 9 - 6. What products are obtained when...Ch. 9 - 7. Which of the glycerophospholipid head groups...Ch. 9 - Prob. 8ECh. 9 - 9. Identify the hormone molecule shown here. What...Ch. 9 - 10. Draw the structure of the fatty acid amide...
Ch. 9 - Prob. 11ECh. 9 - Prob. 12ECh. 9 - Prob. 13ECh. 9 - Prob. 14ECh. 9 - Prob. 15ECh. 9 - Prob. 16ECh. 9 - 17. (a) How many turns of an ? helix are required...Ch. 9 - 18. The distance between successive C? atoms in a...Ch. 9 - 19. Are the following lipid samples likely to...Ch. 9 - 20. In order to consume and dispose of dying...Ch. 9 - 21. Sphingosine-1-phosphate can serve as a cell...Ch. 9 - 22. Lipids known as sulfatides occur in cells of...Ch. 9 - 23. In some autoimmune diseases, an individual...Ch. 9 - 24. Most hormones, such as peptide hormones, exert...Ch. 9 - 25. E. coli outer membranes include a component...Ch. 9 - 26. Archaebacteria and Eubacteria produce...Ch. 9 - 27. Shigella bacteria cause severe diarrhea by...Ch. 9 - 28. The inner membranes of mitochondria are rich...Ch. 9 - 29. Describe the labeling pattern of glycophorin....Ch. 9 - 30. Identify a 20-residue segment that could form...Ch. 9 - 31. Macropinocytosis is a variation of endocytosis...Ch. 9 - 32. Explain why the action of phospholipase A2 on...Ch. 9 - 33. Predict the effect of a mutation in signal...Ch. 9 - 34. Explain why a drug that interferes with the...Ch. 9 - 35. The symmetries of oligomeric integral proteins...Ch. 9 - Prob. 36CQCh. 9 - MORE TO EXPLORE Some of the more unusual membrane...
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