Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 8.6, Problem 1CR
Bacteriophage T4 lacks its own RNA polymerase. How do T4 genes get expressed or converted to mRNA? What host barriers must be broken before release of virions from the host cell?
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What is the difference between the lytic and lysogenic cycle of bacteriophages?
In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change
The host cell is not killed in the lysogenic cycle
The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm
In the lysogenic cycle, penetration is achieved through phagocytosis
During the lysogenic cycle of lambda (1) phage
the phage genome is integrated in the host chromosome where it is passed on to daughter cells through binary fission.
the phage DNA is copied and accumulates in the cytoplasm. Then a trigger causes capsid proteins to be made and phages are assembled.
the product of one prophage gene is made and it activates most of the other prophage genes.
the viral genes immediately turn the host cell into a lambda-producing factory. Then the host lyses.
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Why does phage T4 need a lysozyme-like protein in order toinfect its host, and what part of T4 enters the host cytoplasm?
Chapter 8 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 8.1 - How does a virus differ from a cell?Ch. 8.1 - Why does a virus need a host cell?Ch. 8.1 - Compared with cells, what is unusual about viral...Ch. 8.1 - Once inside a host prokaryotic cell, what are the...Ch. 8.2 - Distinguish between a capsid and a capsomere. What...Ch. 8.2 - What is the difference between a naked virus and...Ch. 8.2 - What kinds of enzymes can be found within the...Ch. 8.2 - Where does the envelope surrounding animal viruses...Ch. 8.3 - What is packaged into capsids during maturation?Ch. 8.3 - Explain the term burst size.
Ch. 8.3 - Prob. 3MQCh. 8.3 - Why does a one-step growth curve differ in shape...Ch. 8.4 - What is meant by a viral titer?Ch. 8.4 - What is a plaque-forming unit?Ch. 8.4 - What is meant by the term plating efficiency?Ch. 8.4 - Describe the events that occur on an agar plate...Ch. 8.5 - How does attachment contribute to virushost...Ch. 8.5 - Prob. 2MQCh. 8.5 - Prob. 3MQCh. 8.5 - What is required for a bacteriophage T4 virion to...Ch. 8.6 - Prob. 1MQCh. 8.6 - Give one example each of T4 early, middle, and...Ch. 8.6 - What is required to package the T4 genome into its...Ch. 8.6 - Bacteriophage T4 lacks its own RNA polymerase. How...Ch. 8.7 - What is a lysogen and what is a prophage?Ch. 8.7 - How does DNA replication in lambda differ from...Ch. 8.7 - What commits lambda to the lytic versus the...Ch. 8.7 - What enzyme is required to form a prophage, and...Ch. 8.8 - Prob. 1MQCh. 8.8 - What is the difference between a persistent and a...Ch. 8.8 - Prob. 3MQCh. 8.8 - Why can it be said that the retrovirus genome is...Ch. 8 - What causes the viral plaques that appear on a...Ch. 8 - The promoters on genes encoding early proteins in...Ch. 8 - Under some conditions, it is possible to obtain...
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- Which of the following statements describes the lysogenic cycle of lambda (A) phage? After infection, the viral genes immediately turn the host cell into a lambda-producing factory, and the host cell then lyses. Most of the prophage genes are activated by the product of a particular prophage gene. The phage DNA is incorporated into the bacteria DNA The phage DNA is copied and exits the cell as a phage. The phage DNA get destructed by the host bacteria DNAarrow_forwardMost phages that use DNA to propagate work by hijacking host bacterial machinery including RNA Polymerase. Scientists found that treatment of jumbo bacteriophage PhiKZ with Rifampicin did not stop the phage from propagating. What is allowing for the survival of this phage?arrow_forwardphage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnductionarrow_forward
- You are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forwardHow is host cell lysis triggered after bacteriophage invasion? What are the outcomes to bacterial host physiology if the bacteriophage undergoes the lysogenic cycle?arrow_forwarda. Which type of viruses can be directly used for translation? a) dsRNA b) Positive ssRNA c) Retroviruses d) Negative ssRNA b. An antiviral drug specifically modifies viral receptors on a eukaryotic host cell. How might this affect the viral reproductive cycle? It would enhance the process of viral apoptosis It would result in the degeneration of the viral capsid It would facilitate the process of viral endocytosis It would prevent the virus from attaching to the host cellarrow_forward
- What are the advantages and disadvantages to bacteriophages of the lytic and lysogenic reproductive strategies?arrow_forwardWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forwardbriefly describe four explanations why E. coli phages can only infect E. coli cells, but not other bacteria or human cells.arrow_forward
- In what major way does transcription of phage DNA differ inphages T4 and T7?arrow_forwardDraw a diagram of the one-step growth curve ofvirus replication, and briefly explain why the growthcurve differs from a typical bacterial growth curve.arrow_forwardWhat type of infection is caused by a temperate phage?arrow_forward
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