Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Textbook Question
Chapter 8.1, Problem 2MQ
- Why does a virus need a host cell?
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 8 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 8.1 - How does a virus differ from a cell?Ch. 8.1 - Why does a virus need a host cell?Ch. 8.1 - Compared with cells, what is unusual about viral...Ch. 8.2 - Distinguish between a capsid and a capsomere. What...Ch. 8.2 - What is the difference between a naked virus and...Ch. 8.2 - What kinds of enzymes can be found within the...Ch. 8.3 - What is packaged into capsids during maturation?Ch. 8.3 - Explain the term burst size.Ch. 8.3 - Prob. 3MQCh. 8.4 - What is meant by a viral titer?
Ch. 8.4 - What is a plaque-forming unit?Ch. 8.4 - What is meant by the term plating efficiency?Ch. 8.5 - How does attachment contribute to virushost...Ch. 8.5 - Prob. 2MQCh. 8.5 - What part of phage T4 enters the host cytoplasm?
Ch. 8.6 - Prob. 1MQCh. 8.6 - Prob. 2MQCh. 8.6 - Prob. 3MQCh. 8.7 - Give one example each of T4 early, middle, and...Ch. 8.7 - Prob. 2MQCh. 8.7 - What is required to package the T4 genome into its...Ch. 8.8 - What is a lysogen and what is a prophage?Ch. 8.8 - How does DNA replication in lambda differ from...Ch. 8.8 - What commits lambda to the lytic versus the...Ch. 8.9 - Prob. 1MQCh. 8.9 - Prob. 2MQCh. 8.9 - Prob. 3MQCh. 8.10 - Prob. 1MQCh. 8.10 - Prob. 2MQCh. 8.10 - Prob. 3MQCh. 8.11 - What type of bacteriophages are most common in the...Ch. 8.11 - Prob. 2MQCh. 8.11 - Prob. 3MQCh. 8 - Prob. 1RQCh. 8 - Prob. 2RQCh. 8 - Prob. 3RQCh. 8 - Why does a one-step growth curve differ in shape...Ch. 8 - Describe the events that occur on an agar plate...Ch. 8 - Prob. 6RQCh. 8 - What is required for a bacteriophage T4 virion to...Ch. 8 - Prob. 8RQCh. 8 - Prob. 9RQCh. 8 - Bacteriophage T4 lacks its own RNA polymerase. How...Ch. 8 - Prob. 11RQCh. 8 - Prob. 12RQCh. 8 - Prob. 13RQCh. 8 - Prob. 14RQCh. 8 - Why can it be said that the retrovirus genome is...Ch. 8 - Prob. 16RQCh. 8 - Prob. 17RQCh. 8 - What causes the viral plaques that appear on a...Ch. 8 - The promoters on genes encoding early proteins in...Ch. 8 - Under some conditions, it is possible to obtain...Ch. 8 - Prob. 4AQ
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