(a) Interpretation: Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified. Concept introduction: Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1 s t step and helps in the catalysis of base whereas it act as general acid catalyst in another step. Glutamine consists of carboxyl group which attacks the hydroxyl of Zn 2 + and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase. Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

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Answer 1

Question

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

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Fura-2 Fluorescence (Arbitrary Unit) 4500 4000 3500 3000 2500 2000 1500 1000 500 [Ca2+]=2970nM, 25°C [Ca2+] 2970nM, 4°C [Ca2+]=0.9nM, 25°C [Ca2+] = 0.9nM, 4°C 0 260 280 300 340 360 380 400 420 440 Wavelength (nm) ← < The figure on the LHS shows the excitation spectra of Fura-2 (Em = 510 nm) in 2 solutions with two different Ca2+ ion concentration as indicated. Except for temperature, the setting for excitation & signal acquisition was identical.< ப a) The unit in Y-axis is arbitrary (unspecified). Why? < < b) Compare & contrast the excitation wavelength of the Isosbestic Point of Fura-2 at 25 °C & 4 °C. Give a possible reason for the discrepancy. < c) The fluorescence intensity at 25 °C & 4 °C are different. Explain why with the concept of electronic configuration. <

Answer 2

Question

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

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Answer 3

Question

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

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Answer 4

Question

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

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Answer 5

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

Answer 6

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Answer 7

Chapter 8, Problem 9P

Interpretation Introduction

(a) Interpretation:

Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1^s^t step and helps in the catalysis of base whereas it act as general acid catalyst in another step.

Glutamine consists of carboxyl group which attacks the hydroxyl of Zn²+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.

Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.

Answer 8

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

Answer 9

Interpretation Introduction

(b) Interpretation:

Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.

Concept introduction:

Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 8 - Prob. 1P Ch. 8 - The enzyme urease catalyzes the hydrolysis of urea...Ch. 8 - An enzyme contains an active site aspartic acid...Ch. 8 - The folding and unfolding rate constants for a...Ch. 8 - In some reactions, in which a protein molecule is...Ch. 8 - Would you expect an “enzyme” designed to bind to...Ch. 8 - The initial rate for an enzyme-catalyzed reaction...Ch. 8 - a. If the total enzyme concentration in Problem 7...Ch. 8 - Prob. 9P Ch. 8 - Prob. 10P

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