Concept explainers
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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- The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forward
- Restriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forwardWhich of the following natural cellular process does PCR use to amplify a target DNA? A. Gene Expression B. DNA symthesis C. Transcription of DNA D. Translationarrow_forwardSome bacteria, through natural selection, have acquired some extremely potent enzymes that destroy viral DNA, thereby preventing the bacterial cell from becoming infected with the virus. These enzymes are called: Select one: O a. DNA polymerases O b. DNA ligases c. restriction endonucleases O d. restriction ligasesarrow_forward
- The restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forwardWhy do prokaryotes produce restriction enzymes? A. Restriction enzymes are produced to enable bacteria to repair lesions in its DNA B. Restriction enzymes enable prokaryotes to assimilate genes or fragments of DNA from its environment C. Restriction enzymes excise phage DNA from prokaryotes thereby protecting them from lysis. D. Restriction enzymes help unwind DNA and facilitate DNA replication in prokaryotes E. Restriction enzymes are necessary for editing DNA during replication.arrow_forwardWhich of the following sequences, when combined with itscomplement, would be clipped by a restriction endonuclease?a. ATCGATCGTAGCTA b. AAGCTTCGAA c. GAATTC d. ACCATTGGAarrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardWhich of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forwardWhich of the following methods use(s) a labeled nucleic acidprobe, such as a labeled fragment of DNA?a. Site-directed mutagenesis c. Western blottingb. Northern blotting d. Both a and barrow_forward
- Concepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College