Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 5MC
Summary Introduction
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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Which technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro?
a.
The CRISPR-Cas9 system
b.
PCR-based random mutagenesis
c.
PCR-based site-specific mutagenesis
d.
RT-PCR
Which process consists of modifying an individual’s genome?
a.
DNA profiling
b.
SNPing
c.
genetic engineering
d.
DNA hybridization
e.
PCR
Which technology facilitates the production of novel DNA molecules by combining sequences from DNA from two different organisms?
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C. Polymerase chain reaction
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Chapter 8 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- Which lab technique can you use to verify? a.Nucleic Acid Purification b. PCR c.SNP Variationarrow_forwardWhat repair system will have an issue functioning properly if DNA glycosylase loses its function? A. Photoreactivation repair B. Base excision repair C. Nucleotide excision repair D. SOS repairarrow_forwardwhich process releases the DNA into the ionic medium? a. homogenization b. deproteination c. precipitation d. all are correctarrow_forward
- Which type of mutation produces the same protein despite a change in the DNA? A. nonsense B. missense C. silent D. frameshiftarrow_forwardWhich would be most useful in the process of lysing a bacterium so that it's DNA could be collected and studied? A. Lysozyme B. Penicillin C Pymoxin B D. Tetracyclinesarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forward
- What is the role of Sodum Dodecyl Sulffate in DNA isolation? A. Precipitate DNA B Make the solution more viscous C Destroy the cell membrane D Maintain ionic strengtharrow_forwardWhich process is most likely to result in a gene providing antibiotic resistance being found in a viral genome? A. Conjugation B. Binary fission C. Transduction D. Transformationarrow_forwardWhich DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b. 3,560 bp c. 5 kb d. 1,500 bparrow_forward
- A scientist wants to study the cytochrome c gene of a fruit fly. The DNA for the gene is extracted and subjected to several treatments. If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forwardMatch the process to the step in producing a transgenic plant using the Ti plsmid method. 45. A gene is isolated and the process starts. 46. The recombinant DNA has this so it can be found later. 47. The plasmid enters here. 48. A. tumefaciens infects target organism. 49. The selectable marker allows transgenic plants to survive antibiotic. 50. Transgenic plant cells are isolated. 51. Transgenic plants are prepared for life in the soil. Kanamycin is added to culture. b. The isolated transgenic cells are grown into multicellular organisms. C. Ti plasmid picks up gene of interest. d. Bacterium Agrobacterium tumefaciens takes this up. A selectable marker makes the cell resistant to kanamycin. a. e. f. Antibiotics kill any remaining bacteria. g. The host plant is selected and exposed to the new DNA.arrow_forwardWhat is the role of CTAB in DNA isolation? A. Neutralize negative charge of DNA B Precipitate DNA C Remove polyphenols and tannins D Precipitate polysaccharidesarrow_forward
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