Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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Microbiology with Diseases by Body System (5th Edition)
- Which lab technique can you use to verify? a.Nucleic Acid Purification b. PCR c.SNP Variationarrow_forwardWhat repair system will have an issue functioning properly if DNA glycosylase loses its function? A. Photoreactivation repair B. Base excision repair C. Nucleotide excision repair D. SOS repairarrow_forwardwhich process releases the DNA into the ionic medium? a. homogenization b. deproteination c. precipitation d. all are correctarrow_forward
- Which type of mutation produces the same protein despite a change in the DNA? A. nonsense B. missense C. silent D. frameshiftarrow_forwardDNA polymerases that corrects errors and mismatches in DNA is called? A. Post replication repair B. Base excision repair C. Nucleotide excision repair D. Proofreadingarrow_forwardWhich would be most useful in the process of lysing a bacterium so that it's DNA could be collected and studied? A. Lysozyme B. Penicillin C Pymoxin B D. Tetracyclinesarrow_forward
- What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? a.DNA ligase seals any gaps remaining between bases of new DNA. b.Primers base-pair with the exposed single DNA strands. c.Repair enzymes correct potential mutations in the DNA sequence. d.Enzymes unwind and separate the two strands of DNA.arrow_forwardWhat is the role of Sodum Dodecyl Sulffate in DNA isolation? A. Precipitate DNA B Make the solution more viscous C Destroy the cell membrane D Maintain ionic strengtharrow_forward
- Which process is most likely to result in a gene providing antibiotic resistance being found in a viral genome? A. Conjugation B. Binary fission C. Transduction D. Transformationarrow_forwardWhich process involves the deliberate alteration of an organism’s genetic material?a. bioremediation c. decompositionb. biotechnology d. recombinant DNA technologyarrow_forwardA mutation in which of these proteins will lead to increased mutations in all daughter cells? A. helicase B. topoisomerase C. ligase D. DNA polymerasearrow_forward
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