Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Ch.23 How is Salmonella able to cross from the intestines into the blood? A. it is so small that it can squeeze between intestinal cells B. it secretes a toxin that induces its uptake into intestinal epithelial cells C. it secretes enzymes that create perforations in the intestine D. it can get into the blood only if the bacteria are deposited directly there, that is, through a puncture — Which virus is associated with liver cancer? A. hepatitis A B. hepatitis B C. hepatitis C D. both hepatitis B and C — explain your answer thoroughly

Ch.21 What causes patients infected with the yellow fever virus to turn yellow (jaundice)? A. low blood pressure and anemia B. excess leukocytes C. alteration of skin pigments D. liver damage in final stage of disease — What is the advantage for malarial parasites to grow and replicate in red blood cells? A. able to spread quickly B. able to avoid immune detection C. low oxygen environment for growth D. cooler area of the body for growth — Which microbe does not live part of its lifecycle outside humans? A. Toxoplasma gondii B. Cytomegalovirus C. Francisella tularensis D. Plasmodium falciparum — explain your answer thoroughly

Ch.22 Streptococcus pneumoniae has a capsule to protect it from killing by alveolar macrophages, which kill bacteria by… A. cytokines B. antibodies C. complement D. phagocytosis — What fact about the influenza virus allows the dramatic antigenic shift that generates novel strains? A. very large size B. enveloped C. segmented genome D. over 100 genes — explain your answer thoroughly