Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 1MTF
Modified True/False
1. ________ Restriction enzymes inhibit the movement of DNA at specific sites.
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Activity 2. You COMPLETE Me!
Objective: Identify the methods used for inserting plasmids into the host cells.
What you need: pen and paper
What to do: On a separate sheet of paper, copy and complete the key points of the methods used
to introduce plasmids into the host cells. Choose your answers from the box
gene gun
biolistics
electric shock
mammalian cells
plasmid insertion by Heat Shock Treatment
increase the pore sizes of their plasma membranes
(2)
competent
plasmid DNA
Heat Shock
electroporation
There are three methods on introducing plasmids into the host cells namely: (1)_
(3)
Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues.
Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a
The pretreatment is said to make the cells (6).
pretreatment procedure to (5)
the introduction of the (7)_
plasmid at about 4°C for about
30 minutes and the plasmids concentrate near the cells during
After the pretreatment, the cells are incubated with…
Pick the correct answer
1. White or blue
2. resistant or sensitive
Pipetting
1. Explain why a micropipettor is an important instrument in biochemistry labs.
2. Describe the basic components (and function) of a micropipettor.
Bacterial Techniques
1. Define the following.
(a) Serial Dilution
(b) Streak Plating
(c) Spread Plating
Transformation
1. Define bacterial transformation and explain why it is an important method in
biochemistry labs.
2. Describe (figure, narrative) a plasmid and describe the basic components of a
plasmid.
3. What role does CaCl2 play in bacterial transformation?
4. What role does heat shock play in bacterial transformation?
Plasmid Isolation
1. Describe the roles the following play in plasmid purification.
(a) Lysis Buffer
(b) Neutralization Buffer
(c) Elution Buffer
Chapter 8 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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