To answer: Recognition and restriction of DNA sequence by Hha I. Introduction: Restriction enzymes recognize and cut DNA nucleotide sequences at the specific sites is called restriction site. Most of the restriction sites are palindromic sequences (the sequence can be read both forward or backward). The hundreds of restriction enzymes are classified into two groups based on their types of cuts (sticky or blunt ends) on DNA sequences. Eco RI cuts DNA sequence and both ends have overhanging ssDNA pieces (staggered cut) is called sticky ends. Hind II and Sma I cut dsDNA at the same point and produced blunt ends. In bacteria, restriction enzymes are used for their protection from phages, which are cleaved by restriction enzymes into nonfunctional nucleotide pieces. However, bacterial DNAs are protected by methylation mechanism. Restriction enzymes are mostly denoted by three or more letters which indicates their genus and bacterial sources.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 5CT

Summary Introduction

To answer:

Recognition and restriction of DNA sequence by HhaI.

Introduction:

Restriction enzymes recognize and cut DNA nucleotide sequences at the specific sites is called restriction site. Most of the restriction sites are palindromic sequences (the sequence can be read both forward or backward). The hundreds of restriction enzymes are classified into two groups based on their types of cuts (sticky or blunt ends) on DNA sequences. EcoRI cuts DNA sequence and both ends have overhanging ssDNA pieces (staggered cut) is called sticky ends. HindII and SmaI cut dsDNA at the same point and produced blunt ends. In bacteria, restriction enzymes are used for their protection from phages, which are cleaved by restriction enzymes into nonfunctional nucleotide pieces. However, bacterial DNAs are protected by methylation mechanism. Restriction enzymes are mostly denoted by three or more letters which indicates their genus and bacterial sources.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Answer number seven do what it says.

Which of the following is the process that is "capable of destroying all forms of microbial life"? Question 37 options: Surgical scrub Sterilization Chemical removal Mechanical removal

After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.) Data Table 1Stage Totals PercentInterphase Mitosis: Prophase Metaphase Anaphase Telophase Cytokinesis Totals 100 100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…