Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 8MC
Summary Introduction
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them to recipient organisms after genetic manipulation. The main tools involved in the techniques are
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_____________ are sequences of identical DNA when it's read from the 5' to 3' direction on one strand and the 5' to 3' direction on the other strand.
a. Palindromes
b. snRNPs
c. RFLPs
d. Restriction fragments
e. Restriction endonucleases
Match the method with the appropriate enzyme.___ PCR a. Taq polymerase___ cutting DNA b. DNA ligase___ cDNA synthesis c. reverse transcriptase___ DNA sequencing d. restriction enzyme___ pasting DNA e. DNA polymerase (not Taq)
DNA can be separated based on fragment size using ________.
a.
restriction enzymes
b.
gel electrophoresis
c.
PCR
Chapter 8 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- A DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forwardA DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forwardRNA can be used as a template for the production of DNA through the action of ____. a. ligase b. DNA polymerase c. reverse transcriptase d. restriction endonuclease e. RNA polymerasearrow_forward
- Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forwardEnzymes used to cut genes in recombinant DNA research are known as ____. a. DNA polymerases b. transcriptases c. replicases d. ligases e. restriction enzymesarrow_forwardin Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the amphibian DNA _______. a. the same restrictions enzyme, so that the the restriction site are identical in the DNA of each species b. different restriction enzymes, so that the genes outside the restriction site are maintained c. the same restriction enzymes, to ensure that the newly formed DNA can replicate d. different restriction enzymes, to ensure that the newly introducted genes are maintained in the bacterial DNAarrow_forward
- Match each enzyme name in the left column with the correct descriptive phrase in the right column. a. Topoisomerase II b. DNA ligase c. DNA polymerase y d. Reverse transcriptase i. Catalyzes most nucleotide incorporations in bacterial DNA replication ii. Cleaves RNA in a DNA-RNA hybrid molecule e. DNA polymerase I f. DNA polymerase II iii. Uses a tRNA primer in synthesis of retroviral DNA iv. Acts through an adenylylated DNA inter- mediate v. Catalyzes formation of a double-strand DNA break vi. Catalyzes mitochondrial DNA replicationarrow_forwardPlease explain right answerarrow_forwardWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IVarrow_forward
- RFLP analysis involves the following, except ________ . a. more than one restriction sites b.gene therapy c.intercalation of dye d.electrophoresis e.restrictionarrow_forwardThe anchor used to start a new DNA strand is called a ________. a. trigger b. first c. starterarrow_forwardGMOs are created by ________. a. generating genomic DNA fragments with restriction endonucleases b. introducing recombinant DNA into an organism by any means c. overexpressing proteins in E. coli d. all of the abovearrow_forward
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