Concept explainers
To answer:
Similarity between the results of Southern blot and DNA microarray.
Introduction:
DNA microarray is composed of single-stranded DNA molecules (ssDNA) as cDNA or genomic DNA, which are attached to the solid substrate such as glass slide, a nylon membrane, silicon chips, or other microarray substrates. It is also called DNA chip as it contains a collection of thousands of various DNA molecules at a specific site on the array. The single array may contain DNA molecules from one or more species. The labeled (tagged with fluorescent) DNA sample is washed over the DNA microarray and the DNA molecule binds to its complementary DNA sequence on the array. The presence of targeted DNA sequence is identified by the development of fluorescence. This technique is used to find DNA or RNA in a sample and to evaluate the expression of genes.
Want to see the full answer?
Check out a sample textbook solutionChapter 8 Solutions
Microbiology with Diseases by Body System (5th Edition)
- I understand that microarrays are being used to define the molecular abnormality and the prognosis in some patients with leukaemia. What are microarrays?arrow_forwardWould it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?arrow_forwardIs this DNA extraction procedure needed/necessary for COVID19 screening? If not, what is its counterpart? Describe the procedure done.arrow_forward
- What are the principles of Chelex extraction and solid-phase DNA extraction methods?arrow_forwardAll of the following pieces of information are expected to be obtained directly from Western Blot analysis, except: An estimation of the fusion protein’s size Relative quantity of the fusion protein compared to other samples The amino acid sequence of the fusion protein If the fusion protein is present in the samplearrow_forwardWhat are the main procedures involved in a western blot?arrow_forward
- What are the advantages of molecular diagnostics over conventional methods?arrow_forwardDescribe a method of labeling a DNA probe. Explain how this probe maybe detected after DNA hybridizationarrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forward