Chapter 8, Problem 31P

Using recombinant DNA techniques (which will be described in Chapter 9), it is possible to take the DNA of a gene from any source and place it on a chromosome in the nucleus of a yeast cell. When you take the DNA for a human gene and put it into a yeast cell chromosome, the altered yeast cell can make the human protein. But when you remove the DNA for a gene normally present on yeast mitochondrial chromosomes and put it on a yeast chromosome in the nucleus, the yeast cell cannot synthesize the correct protein, even though the gene comes from the same organism. Explain. What would you need to do to ensure that such a yeast cell could make the correct protein?