Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
8th Edition
ISBN: 9780134015187
Author: John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher: PEARSON
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Chapter 7.3, Problem 7.1CIAP
Interpretation Introduction
Interpretation:
Whether
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 7 Solutions
Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
Ch. 7.1 - Classify each of the following as having potential...Ch. 7.2 - Based on bond energies, which atmospheric gas in...Ch. 7.3 - Prob. 7.1CIAPCh. 7.3 - How many Calories (i.e., kilocalories) are in a...Ch. 7.3 - Prob. 7.3PCh. 7.3 - Prob. 7.4PCh. 7.3 - Prob. 7.5PCh. 7.3 - Prob. 7.6PCh. 7.4 - Prob. 7.7PCh. 7.4 - Prob. 7.8KCP
Ch. 7.5 - The reaction between iron and oxygen to form rust...Ch. 7.6 - Prob. 7.10PCh. 7.8 - Prob. 7.11PCh. 7.8 - Prob. 7.12PCh. 7.8 - Prob. 7.13PCh. 7.8 - The following diagrams represent two similar...Ch. 7.9 - Prob. 7.3CIAPCh. 7.9 - Prob. 7.4CIAPCh. 7.9 - Is the yield of SO3 at equilibrium favored by a...Ch. 7.9 - Prob. 7.16PCh. 7.9 - Prob. 7.17PCh. 7 - What are the signs of H, S, and G for the...Ch. 7 - Prob. 7.19UKCCh. 7 - Prob. 7.20UKCCh. 7 - Two curves are shown in the following energy...Ch. 7 - Prob. 7.22UKCCh. 7 - Prob. 7.23APCh. 7 - Prob. 7.24APCh. 7 - Prob. 7.25APCh. 7 - Prob. 7.26APCh. 7 - Acetylene (H C C H) is the fuel used in welding...Ch. 7 - Prob. 7.28APCh. 7 - Prob. 7.29APCh. 7 - Prob. 7.30APCh. 7 - Prob. 7.31APCh. 7 - Prob. 7.32APCh. 7 - Prob. 7.33APCh. 7 - Prob. 7.34APCh. 7 - Why are most spontaneous reactions exothermic?Ch. 7 - Prob. 7.36APCh. 7 - Prob. 7.37APCh. 7 - Prob. 7.38APCh. 7 - Prob. 7.39APCh. 7 - Prob. 7.40APCh. 7 - Prob. 7.41APCh. 7 - Prob. 7.42APCh. 7 - Prob. 7.43APCh. 7 - Prob. 7.44APCh. 7 - Prob. 7.45APCh. 7 - Prob. 7.46APCh. 7 - Prob. 7.47APCh. 7 - Prob. 7.48APCh. 7 - What is meant by the term chemical equilibrium?...Ch. 7 - Prob. 7.50APCh. 7 - Prob. 7.51APCh. 7 - Prob. 7.52APCh. 7 - Prob. 7.53APCh. 7 - Prob. 7.54APCh. 7 - Use your answer from Problem 7.53 to calculate the...Ch. 7 - Use your answer from Problem 7.54 to calculate the...Ch. 7 - Prob. 7.57APCh. 7 - Prob. 7.58APCh. 7 - Prob. 7.59APCh. 7 - Prob. 7.60APCh. 7 - Prob. 7.61APCh. 7 - Prob. 7.62APCh. 7 - Prob. 7.63APCh. 7 - Prob. 7.64APCh. 7 - Prob. 7.65APCh. 7 - Prob. 7.66APCh. 7 - For the unbalanced combustion reaction shown, 1...Ch. 7 - Prob. 7.68CPCh. 7 - Prob. 7.69CPCh. 7 - Prob. 7.70CPCh. 7 - Urea is a metabolic waste product that decomposes...Ch. 7 - Prob. 7.72CPCh. 7 - Prob. 7.73CPCh. 7 - Methanol, CH3OH, is used as race car fuel. (a)...Ch. 7 - Prob. 7.75CPCh. 7 - Prob. 7.76CPCh. 7 - Prob. 7.77CPCh. 7 - Prob. 7.78GPCh. 7 - Prob. 7.79GPCh. 7 - Prob. 7.80GP
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- Which features of the curves in Figure 30-2 indicates that the enzyme is not consumed in the overall reaction? ES is lower in energy that E + S and EP is lower in energy than E + P. What does this tell you about the stability of ES versus E + S and EP versus E + P.arrow_forwardLooking at the figure 30-5 what intermolecular forces are present between the substrate and the enzyme and the substrate and cofactors.arrow_forwardprovide short answers to the followings Urgent!arrow_forward
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