(a) Interpretation: The total amount of O 2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated. Concept introduction: Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

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Answer 1

Question

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

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Answer 3

Question

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Expert Solution & Answer

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Answer 4

Question

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Expert Solution & Answer

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Answer 5

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Answer 6

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Answer 7

Chapter 7, Problem 4P

Interpretation Introduction

(a)

Interpretation:

The total amount of O2 that is bound to the myoglobin in human muscle and in sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The ratio of oxygen that is bound to myoglobin to that directly dissolved in the water of sperm whale muscle is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein that is an oxygen and iron containing protein present in the muscle tissue of mammals is known as myoglobin.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 7 - Prob. 1P Ch. 7 - Prob. 2P Ch. 7 - Prob. 3P Ch. 7 - Prob. 4P Ch. 7 - Prob. 5P Ch. 7 - Prob. 6P Ch. 7 - Prob. 7P Ch. 7 - Prob. 8P Ch. 7 - Prob. 9P Ch. 7 - Prob. 10P

Solution Summary: The author explains the amount of myoglobin bound to human muscle and sperm whale muscle.

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