(a) Interpretation: The weight of the hemoglobin contained in an average red cell is to be stated. Concept introduction: Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Question

The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

Answer 1

Question

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

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Answer 3

Question

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

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Answer 4

Question

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

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Answer 5

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 6

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 7

Chapter 7, Problem 2P

Interpretation Introduction

(a)

Interpretation:

The weight of the hemoglobin contained in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The total number of hemoglobin molecules present in an average red cell is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 10

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 11

Interpretation Introduction

(c)

Interpretation:

Whether the hemoglobin concentration in the red cells can be much higher than the observed value or not is to be stated.

Concept introduction:

Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.

Answer 12

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Answer 23

Ch. 7 - Prob. 1P Ch. 7 - Prob. 2P Ch. 7 - Prob. 3P Ch. 7 - Prob. 4P Ch. 7 - Prob. 5P Ch. 7 - Prob. 6P Ch. 7 - Prob. 7P Ch. 7 - Prob. 8P Ch. 7 - Prob. 9P Ch. 7 - Prob. 10P

Solution Summary: The author explains the weight of the hemoglobin contained in an average red cell.

Chapter 7 Solutions