EBK HUMAN ANATOMY & PHYSIOLOGY
1st Edition
ISBN: 9780100659834
Author: AMERMAN
Publisher: YUZU
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Textbook Question
Chapter 6.2, Problem 1QC
What are the two components of bone ECM? How do these components differ?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 6 Solutions
EBK HUMAN ANATOMY & PHYSIOLOGY
Ch. 6.1 - 1. What are the main organs of the skeletal...Ch. 6.1 - 2. What are the primary functions of the skeletal...Ch. 6.1 - 3. Look at Figure 6.2 and classify the following...Ch. 6.1 - 4. Where are compact bone and spongy bone...Ch. 6.1 - What are the two types of bone marrow, and what...Ch. 6.2 - What are the two components of bone ECM? How do...Ch. 6.2 - 2. Compare and contrast the locations and...Ch. 6.2 - Match the following terms with the correct...Ch. 6.3 - 1. How do primary bone and secondary bone...Ch. 6.3 - Walk through the basic steps of intramembranous...
Ch. 6.3 - For each type of ossification: a. Does spongy bone...Ch. 6.3 - 5. Which type of ossification:
a. involves a bone...Ch. 6.4 - What tissue type makes up the epiphyseal plate?Ch. 6.4 - How does bone grow in length from the epiphyseal...Ch. 6.4 - Prob. 3QCCh. 6.4 - Prob. 4QCCh. 6.4 - How is bone growth affected by the sex hormones...Ch. 6.5 - How do bone resorption and bone deposition differ?Ch. 6.5 - Explain how compression, continuous pressure on a...Ch. 6.5 - What role does parathyroid hormone play in calcium...Ch. 6.5 - 4. Walk through the basic steps of fracture...Ch. 6 - Which of the following is not a function of the...Ch. 6 - Match the following terms with the correct...Ch. 6 - Explain the differences between red bone marrow...Ch. 6 - 4. Mark the following statements about bone...Ch. 6 - Prob. 5CYRCh. 6 - 6. The branching pieces of bone in spongy bone...Ch. 6 - 7. Which bones form via intramembranous...Ch. 6 - Of the following statements, identify those that...Ch. 6 - What is the difference between a primary and...Ch. 6 - 10. The part of the epiphysis that does not...Ch. 6 - Long bones grow in length from the: a. diaphyseal...Ch. 6 - 12. Correctly order the following steps of bone...Ch. 6 - Explain the effect that the following hormones...Ch. 6 - Fill in the blanks. Bone deposition is carried out...Ch. 6 - Mark the following statements as true or false. If...Ch. 6 - 16. Which of the following influences bone...Ch. 6 - Correctly order the following steps of fracture...Ch. 6 - 1. Explain why a person who is wheelchair-bound or...Ch. 6 - 2. How could diseases of the kidney, skin, and/or...Ch. 6 - How could a disease that affects primarily...Ch. 6 - It used to be common practice in surgical fracture...Ch. 6 - Explain why young men who take anabolic steroids...Ch. 6 - Prob. 3AYKACh. 6 - Prob. 4AYKB
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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