BIOCHEMISTRY BOOKS ALC&MOD MST/ET PKG
1st Edition
ISBN: 9780134172507
Author: APPLING
Publisher: Pearson Education
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Chapter 6, Problem 9P
a. A protein is found to be a tetramer of identical subunits. Name two symmetries possible for such a molecule. What kinds of interactions (isologous or heterologous) would stabilize each?
b. Suppose a tetramer, like hemoglobin, consists of two each of two types of subunits, a and ß. What is the highest symmetry now possible?
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A protein hypothetically consists of two polypeptide chains with the given sequences in the picture.
Based on the said sequences,
a. Do you think it is possible that interchain disulfide bonds is present in the protein? Explain.
b. Do you think it is possible that intrachain disulfide bonds is present in the protein? Explain.
- (a) A protein is found to be a tetramer of identical subunits. Name
two symmetries possible for such a molecule. What kinds of interac-
tions (isologous or heterologous) would stabilize each?
(b) Suppose a tetramer, like hemoglobin, consists of two each of two
types of chains, a and B. What is the highest symmetry now possible?
A. Where would you expect valine, leucine, and isoleucine to be located in a polypeptide? (Inside or outside)? Explain your answer using the properties of the amino acids listed.
B.How would your answer change, if the amino acids were Lysine and Histidine?
Chapter 6 Solutions
BIOCHEMISTRY BOOKS ALC&MOD MST/ET PKG
Ch. 6 - Prob. 1PCh. 6 - Bovine pancreatic trypsin inhibitor (BPTI; Figure...Ch. 6 - A schematic structure of the subunit of...Ch. 6 - In the protein adenylate kinase, the C-terminal...Ch. 6 - Give two reasons to explain why a proline residue...Ch. 6 - Consider a small protein containing 101 amino acid...Ch. 6 - a. Based on a more conservative answer to Problem...Ch. 6 - The following sequence is part of a globular...Ch. 6 - a. A protein is found to be a tetramer of...Ch. 6 - Under physiological conditions, the protein...
Ch. 6 - Theoretical and experimental measurements show...Ch. 6 - The peptide hormone vasopressin is used in the...Ch. 6 - A protein gives under conditions of buffer...Ch. 6 - A protein gives a single band on SDS get...Ch. 6 - It has been postulated that the normal...Ch. 6 - Prob. 16PCh. 6 - Do you expect a Pro Gly mutation in a...Ch. 6 - Rank the following in terms of predicted rates...
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- 3a) The 3° structure of a protein refers to the protein's overall, 3-dimensional shape in space. This will incorporate any 2° structure the protein has, but is predominantly the result of side-group interactions. Name the type of interaction, if any, that you would expect to see under physiological conditions (an aqueous solution at pH 7.0-7.5) between the following pairs of amino acid side groups. Serine and asparagine: Methionine and lysine: Glutamate and aspartic acid: Alanine and phenylalanine:arrow_forwardThe given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa. Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture). 1: Standard Protein Ladder 2: Protein X solution 3: Protein X solution + β-mercaptoethanol Based on the given gel profile in the picture, can it be concluded that the solution of Protein X is pure? Explain. can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.arrow_forwardA B 10 Refer to the following figure. How many H-bonds would be present in the four base pairs that are indicated in this sequence: C) 8 D) 12 TT T·* сссG | | | | & E diearrow_forward
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