To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained. Concept introduction: Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient. If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode. Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Question

Want to see the full answer?

Answer 1

Question

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

How will you prepare the following buffers? 2.5 L of 1.5M buffer, pH = 10.5 from NH4Cl and NH3

CH₂O and 22 NMR Solvent: CDCl3 IR Solvent: neat 4000 3000 2000 1500 1000 15 [ اند 6,5 9.8 3.0 7.0 6.0 5.0 4.8 3.0 2.0 1.0 9.8 200 100

protons. Calculate the mass (in grams) of H3AsO4 (MW=141.9416) needed to produce 3.125 x 1026

Answer 2

Question

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Answer 6

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Answer 7

Chapter 6, Problem 6.37P

Interpretation Introduction

Interpretation:

To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient 1-12 is to be explained.

Concept introduction:

Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.

If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.

Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 6 - Prob. 6.1P Ch. 6 - Prob. 6.2P Ch. 6 - Prob. 6.3P Ch. 6 - Prob. 6.4P Ch. 6 - Prob. 6.5P Ch. 6 - Prob. 6.6P Ch. 6 - Prob. 6.7P Ch. 6 - Prob. 6.8P Ch. 6 - Prob. 6.9P Ch. 6 - Prob. 6.10P

Solution Summary: The author explains how lysine migrates to anode in an isoelectric focusing experiment with gel of pH gradient 1-12.

Concept explainers

Want to see the full answer?

Chapter 6 Solutions