Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 5P
Explain the importance of the following features in conjugating donor bacteria:
a. the origin of transfer
b. the conjugation pilus
c. homologous recombination
d. the relaxosome
e. relaxase
f. T strand DNA
g. pilin protein
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Students have asked these similar questions
For bacteria that are F+, Hfr, F', and F- answer the following.
a. Describe the state of the F factor.
b. Which of these cells are donors? Which is the recipient?
c. Which of these donors can convert exconjugants to a donor state?
d. Which of these donors can transfer a donor gene to exconjugants?
e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain.
f. Describe a "partial diploid" and how it originates.
The following recombinants are recovered when
conjugation occurs between an a+d+g+ donor and an a
d'g recipient.
at dt g+= 84%
ad g+= 6%
at dgt = 10%
ad g+
= less than 1%
Which gene is in the middle?
a
The genes are located too far apart on the bacterial
genome to determine which gene is in the middle.
d
Insufficient data are provided to answer this
question.
Genetic information can be transferred horizontally from one bacterial cell to another cell of the "same generation" (versus daughter cells), leading to genetic recombination, by all of the following EXCEPT:
A. Gene expression (transcription/translation/protein synthesis)
B. Transformation
C. Conjugation
D. Transduction
E. None of the other four answers (all are mechanisms of “horizontal transfer” genetic recombination)
Chapter 6 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - Prob. 15PCh. 6 - 16. Suppose you have an lysis mutant that maps to...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Five rII partial-deletion mutants are mapped and...Ch. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forwardThe likelihood of co-transformation of two bacterial genes increases: a. As their distance apart on the chromosome increases b. As their distance apart on the chromosome decreases c. As the transducing phage enter the lytic cycle d. As recombination rate decreases e. None of these is correcarrow_forwardThe following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dg+ = 10% ad g+= less than 1% What is the map distance between the a and d genes? 6 map units less than 1 map unit 10 map units 84 map units 74 map unitsarrow_forward
- Which of the following statement about conjugation is TRUE: During F+ to F- mating, the donor becomes F-. During the Hfr to F- transfer, the recipient becomes F+. The whole bacteria genome may get transferred during Hfr mating. During the Hfr to F- transfer, some recipients become recombinants in which transferred fragment recombines with homologous region in the genome.arrow_forwardOrder the following experimental steps to identify auxotrophic mutants in Saccharomyces Cerevisiae that cannot sythesize their own Leucine. an option can be selected more than once. step 1: Step 2: Step 3: Step 4: Step 5: Step 6:arrow_forwardAll of the following apply to Luria and Delbrűck’s mutation theory as tested using E. Coli and T1 phage EXCEPT: A. It was performed using liquid bacterial cultures B. It involved looking for T1-resistant bacteria as an end measure C. It was supported by the finding that the number of final cultures that were T1 phage-resistant were constant from experiment to experiment D. It rejected the possibility of mutations occurring only in response to a T1 phage challenge E. It supported the concept of spontaneous mutation ratesarrow_forward
- Describe the mechanisms of horizontal gene transfer in bacteria, describing the manner in which the DNA can be transferred from one cell to another.Choose a disease or an organism that has a well-documented mechanism of resistance (such as methicillin resistant Staphylococcus aureus - MRSA or tuberculosis) and identify the gene or genes that confer resistance and the method of DNA transfer thought to contribute to its spread. This may be from one organism to another or may simply be passed down from mother to daughter cell due to environmental pressure.arrow_forwardDescribe the events (in words) occurring during conjugation in bacterial cells (using F plasmid transfer)arrow_forwardDefine the following terms: a. transfection b. replicative transposition c. composite transposition d. retrotransposon e. insertional elementarrow_forward
- Below is a diagram of the general structure of the bacteriophagel chromosome. Speculate on the mechanism by which it forms aclosed ring upon infection of the host cell. 5'GGGCGGCGACCT:double@stranded region-3' 3'- double@stranded region:CCCGCCGCTGGA5'arrow_forwardDraw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forwardDetermine the map distances between the genes.arrow_forward
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