Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN: 9781305073951
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 6, Problem 2DAA
Energy Efficiency of Biofuel Production Most of the plant material currently used for biofuel production in the United States consists of food crops—mainly corn, soybeans, and sugarcane. In 2006, David Tilman and his colleagues published the results of a 10-year study comparing the net energy output of various biofuels. The researchers grew a mixture of native perennial grasses without irrigation, fertilizer, pesticides, or herbicides, in sandy soil that was so depleted by intensive agriculture that it had been abandoned. They measured the usable energy in biofuels made from the grasses, and also from com and soy, then measured the energy it took to grow and produce biofuel from each kind of crop (FIGURE 6.18).
FIGURE 6 .18 Energy inputs and outputs of biofuels made from three different crops. One hectare is about 2 .5 acres.
2. Which of the biofuels tested had the highest ratio of energy output to energy input?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 6 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 6 - A cat eats a bird, which ate a caterpillar that...Ch. 6 - Plants use__________ as an energy source to drive...Ch. 6 - Most of the carbon dioxide that plants use for...Ch. 6 - Which of the following statements is incorrect? a....Ch. 6 - Prob. 5SQCh. 6 - When a photosystem absorbs light, __________. a....Ch. 6 - Prob. 7SQCh. 6 - What accumulates inside the thylakoid compartment...Ch. 6 - The atoms in the molecular oxygen released during...Ch. 6 - Prob. 10SQ
Ch. 6 - Prob. 1DAACh. 6 - Energy Efficiency of Biofuel Production Most of...Ch. 6 - Energy Efficiency of Biofuel Production Most of...Ch. 6 - The Calvin-Benson cycle starts when a. light is...Ch. 6 - Which of the following substances does not...Ch. 6 - Closed stomata ________. a. limit gas exchange b....Ch. 6 - In C3 plants, ______ makes sugar production...Ch. 6 - Match each with its most suitable description....Ch. 6 - Prob. 1CTCh. 6 - While gazing into an aquarium, you see bubbles...Ch. 6 - AC3 plant absorbs a carbon radioisotope (as part...Ch. 6 - As you learned in this chapter, cell membranes arc...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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