Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN: 9781305073951
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Textbook Question
Chapter 6, Problem 14SQ
In C3 plants, ______ makes sugar production inefficient when stomata close during the day.
- a. photosynthesis
- b. photolysis
- c. photorespiration
- d. carbon fixation
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 6 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 6 - A cat eats a bird, which ate a caterpillar that...Ch. 6 - Plants use__________ as an energy source to drive...Ch. 6 - Most of the carbon dioxide that plants use for...Ch. 6 - Which of the following statements is incorrect? a....Ch. 6 - Prob. 5SQCh. 6 - When a photosystem absorbs light, __________. a....Ch. 6 - Prob. 7SQCh. 6 - What accumulates inside the thylakoid compartment...Ch. 6 - The atoms in the molecular oxygen released during...Ch. 6 - Prob. 10SQ
Ch. 6 - Prob. 1DAACh. 6 - Energy Efficiency of Biofuel Production Most of...Ch. 6 - Energy Efficiency of Biofuel Production Most of...Ch. 6 - The Calvin-Benson cycle starts when a. light is...Ch. 6 - Which of the following substances does not...Ch. 6 - Closed stomata ________. a. limit gas exchange b....Ch. 6 - In C3 plants, ______ makes sugar production...Ch. 6 - Match each with its most suitable description....Ch. 6 - Prob. 1CTCh. 6 - While gazing into an aquarium, you see bubbles...Ch. 6 - AC3 plant absorbs a carbon radioisotope (as part...Ch. 6 - As you learned in this chapter, cell membranes arc...
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